State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, People's Republic of China.
Bioresour Technol. 2013 May;135:254-61. doi: 10.1016/j.biortech.2012.11.033. Epub 2012 Nov 15.
Sulfuric acid treated corn fiber hydrolysate (SACFH) inhibited cell growth and the production of butanol (4.7±0.2 g/L) by Clostridium beijerinckii IB4 in P2 medium. Optimal medium components were determined using fractional factorial design. NH4HCO3, FeSO4·7H2O and CaCO3 were demonstrated to be significant components in the production of butanol. The Box-Behnken design and a corresponding quadratic model were used to predict medium components (NH4HCO3 1.96 g/L, FeSO4·7H2O 0.26 g/L and CaCO3 3.15 g/L) and butanol yield (9.5 g/L). The confirmation experiment, under the predicted optimal conditions, yielded a butanol level of 9.5±0.1g/L. This study indicates that the Box-Behnken design is an effective approach for screening the optimal medium components required for the production of butanol. It also demonstrates that SACFH, which has high levels of inhibitors such as furan and phenolic compounds, may be used as a renewable carbon source in the production of biofuels.
硫酸处理玉米纤维水解物（SACFH）抑制了丙酮丁醇梭菌 IB4 在 P2 培养基中的细胞生长和丁醇（4.7±0.2 g/L）的生产。使用分阶实验设计确定了最佳培养基成分。NH4HCO3、FeSO4·7H2O 和 CaCO3 被证明是生产丁醇的重要成分。Box-Behnken 设计和相应的二次模型用于预测培养基成分（NH4HCO3 1.96 g/L、FeSO4·7H2O 0.26 g/L 和 CaCO3 3.15 g/L）和丁醇产量（9.5 g/L）。在预测的最佳条件下进行验证实验，得到的丁醇水平为 9.5±0.1g/L。本研究表明，Box-Behnken 设计是筛选生产丁醇所需最佳培养基成分的有效方法。它还表明，SACFH 可能作为一种可再生碳源用于生物燃料的生产，SACFH 含有高水平的抑制剂，如呋喃和酚类化合物。
