State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, PR China.
Bioresour Technol. 2013 May;135:379-85. doi: 10.1016/j.biortech.2012.08.029. Epub 2012 Aug 17.
A Clostridium beijerinckii mutant RT66 with considerable inhibitor-tolerance obtained by continuous culture was used for butanol production from non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (SAHHC). In fed-batch fermentation, 1.8L of diluted SAHHC containing 10 g/L of reducing sugar was provided during the acidogenic phase and 0.2L of concentrated SAHHC containing 300 g/L of reducing sugar was provided during the solventogenic phase. The mutant produced a total amount of solvents of 12.9 g/L, which consisted of 3.1 g/L of acetone, 9.3 g/L of butanol and 0.5 g/L of ethanol. A solvent yield of 0.35 g/g sugar and a productivity of 0.18 g/L h in 72 h were achieved. The remarkable inhibitor-tolerance of C. beijerinckii RT66 demonstrates that this may be an excellent strain for butanol production from ligocellulosic materials.
一株经连续培养获得的具有相当抑制剂耐受性的凝结芽孢杆菌 RT66 被用于从经稀硫酸（SAHHC）处理的玉米纤维半纤维素水解物中生产丁醇。在分批补料发酵中，在产酸阶段提供了 1.8L 含有 10g/L 还原糖的稀释 SAHHC，在溶剂生成阶段提供了 0.2L 含有 300g/L 还原糖的浓缩 SAHHC。突变体共产生了 12.9g/L 的溶剂，其中包括 3.1g/L 的丙酮、9.3g/L 的丁醇和 0.5g/L 的乙醇。在 72 小时内，实现了 0.35g/g 糖的溶剂产率和 0.18g/L/h 的生产率。凝结芽孢杆菌 RT66 的显著抑制剂耐受性表明，它可能是从木质纤维素材料生产丁醇的优良菌株。
