Khan Shahnaz, Khan Hidayatullah, Ali Farman, Ali Nayab, Khan Fahim Ullah, Khan Sami Ullah
a Department of Chemistry , University of Science and Technology , Bannu 28100 , KP , Pakistan.
b Department of Chemistry , Shaheed Benazir Bhutto University , Sheringal, Dir 18000 , KP , Pakistan.
Nat Prod Res. 2016 Jun;30(11):1335-8. doi: 10.1080/14786419.2015.1055743. Epub 2015 Jul 27.
Nelumbo nucifera seeds' essential oil (EO), crude extract and subsequent fractions were evaluated for their DPPH, ABTS and superoxide anion-free radical scavenging and cholinesterase inhibitory activities. The ethyl acetate fraction and EO showed outstanding antioxidant activities with IC50 values of 191, 450 μg/mL (DPPH), 123, 221 μg/mL (ABTS) and 69, 370 μg/mL (superoxide anion). The ethyl acetate fraction and EO also caused significant inhibition of acetylcholinesterase and butyrylcholinesterase with IC50 values of 70 ± 0.6, 64 ± 0.8 and 75 ± 0.3, 58 ± 0.2, in dose-dependent manner. The first ever gas chromatography-mass spectrometry analysis of the EO obtained from N. nucifera seeds resulted in identification of 19 constituents, mainly comprised of oxygenated sesquiterpenes responsible for their promising bioactivity. The crude and fractions revealed the presence of saponins, flavonoids, steroids, alkaloids, terpenoids and cardiac glycosides in phytochemical investigation.
对莲种子的精油（EO）、粗提物及其后续馏分进行了1,1-二苯基-2-三硝基苯肼（DPPH）、2,2'-联氮-二（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）和超氧阴离子自由基清除以及胆碱酯酶抑制活性的评估。乙酸乙酯馏分和EO表现出出色的抗氧化活性，其半抑制浓度（IC50）值分别为191、450μg/mL（DPPH），123、221μg/mL（ABTS）和69、370μg/mL（超氧阴离子）。乙酸乙酯馏分和EO还对乙酰胆碱酯酶和丁酰胆碱酯酶产生了显著抑制作用，其IC50值分别为70±0.6、64±0.8和75±0.3、58±0.2，呈剂量依赖性。首次对莲种子获得的EO进行气相色谱-质谱分析，鉴定出19种成分，主要由含氧倍半萜组成，这是其具有良好生物活性的原因。在植物化学研究中，粗提物和馏分显示出含有皂苷、黄酮类化合物、甾体、生物碱、萜类化合物和强心苷。
