Sanmukh Swapnil, Khairnar Krishna, Paunikar Waman, Lokhande Satish
Environmental Virology Cell, National Environmental Engineering Research Institute (NEERI), Nagpur, Maharashtra, 440020, India.
Analytical Instrumentation Division (AID), CSIR-NEERI, Nehru Marg, Maharashtra, Nagpur-440020, India.
F1000Res. 2015 Jun 1;4:138. doi: 10.12688/f1000research.6031.1. eCollection 2015.
The bacteria and their phages are the most abundant constituents of the aquatic environment, and so represent an ideal model for studying carbon regulation in an aquatic system. The microbe-mediated interconversion of bioavailable organic carbon (OC) into dissolved organic carbon (DOC) by the microbial carbon pump (MCP) has been suggested to have the potential to revolutionize our view of carbon sequestration. It is estimated that DOC is the largest pool of organic matter in the ocean and, though a major component of the global carbon cycle, its source is not yet well understood. A key element of the carbon cycle is the microbial conversion of DOC into inedible forms. The primary aim of this study is to understand the phage conversion from organic to inorganic carbon during phage-host interactions. Time studies of phage-host interactions under controlled conditions reveal their impact on the total carbon content of the samples and their interconversion of organic and inorganic carbon compared to control samples. A total organic carbon (TOC) analysis showed an increase in inorganic carbon content by 15-25 percent in samples with bacteria and phage compared to samples with bacteria alone. Compared to control samples, the increase in inorganic carbon content was 60-70-fold in samples with bacteria and phage, and 50-55-fold for samples with bacteria alone. This study indicates the potential impact of phages in regulating the carbon cycle of aquatic systems.
细菌及其噬菌体是水生环境中最丰富的成分，因此是研究水生系统中碳调节的理想模型。微生物碳泵（MCP）介导的生物可利用有机碳（OC）向溶解有机碳（DOC）的微生物间相互转化，被认为有可能彻底改变我们对碳固存的看法。据估计，DOC是海洋中最大的有机物库，尽管它是全球碳循环的主要组成部分，但其来源尚未得到很好的理解。碳循环的一个关键要素是微生物将DOC转化为不可食用的形式。本研究的主要目的是了解噬菌体-宿主相互作用过程中噬菌体从有机碳到无机碳的转化。在受控条件下对噬菌体-宿主相互作用的时间研究揭示了它们对样品总碳含量的影响，以及与对照样品相比它们对有机碳和无机碳的相互转化。总有机碳（TOC）分析表明，与仅含细菌的样品相比，含有细菌和噬菌体的样品中无机碳含量增加了15%-25%。与对照样品相比，含有细菌和噬菌体的样品中无机碳含量增加了60-70倍，仅含细菌的样品增加了50-55倍。这项研究表明了噬菌体在调节水生系统碳循环方面的潜在影响。
