Ksiaa Feryel, Allous Asma, Ziadi Sonia, Mokni Moncef, Trimeche Mounir
Department of Pathology, Centre Hôpital Universitaire Farhat Hached, Sousse, Tunisia.
J BUON. 2015 May-Jun;20(3):762-9.
Several reports have indicated the presence of JC polyomavirus (JCV) in many human tumors, including colorectal cancers (CRCs). The presence of JCV infection in CRC patients has not been investigated in African countries.
We examined the prevalence and the biological significance of JCV in Tunisian CRC patients. The presence of JCV was assessed by polymerase chain reaction (PCR) in a series of 105 CRCs and 89 paired non-tumor colonic mucosa samples from Tunisian patients. Results were correlated with the clinicopathological features and immunohistochemical expression of β-catenin, p53, and the proliferation marker Ki-67.
JCV DNA was detected in 58.1% (61/105) of CRC and in only 14.6% (13/89) of paired non tumor colonic mucosa samples (p=0.03). The presence of JCV was significantly correlated with tumor differentiation (p=0.03). Moreover, JCV presence was significantly correlated with nuclear accumulation of β-catenin (p=0.008) and p53 accumulation (p=0.0001). Multivariate logistic regression analysis showed that tumor differentiation, β-catenin and p53 accumulation were independent parameters significantly associated with the presence of JCV in CRC (p=0.04; p=0.05; p=0.001, respectively).
We support a role of JCV in colorectal carcinogenesis in Tunisian patients, especially of well differentiated morphology.
多项报告表明，包括结直肠癌（CRC）在内的许多人类肿瘤中存在JC多瘤病毒（JCV）。非洲国家尚未对CRC患者中JCV感染的情况进行调查。
我们研究了突尼斯CRC患者中JCV的患病率及其生物学意义。通过聚合酶链反应（PCR）对105例突尼斯患者的CRC样本以及89例配对的非肿瘤结肠黏膜样本进行JCV检测。结果与临床病理特征以及β-连环蛋白、p53和增殖标志物Ki-67的免疫组化表达相关。
在58.1%（61/105）的CRC样本中检测到JCV DNA，而在配对的非肿瘤结肠黏膜样本中仅14.6%（13/89）检测到（p = 0.03）。JCV的存在与肿瘤分化显著相关（p = 0.03）。此外，JCV的存在与β-连环蛋白的核内积聚（p = 0.008）和p53积聚（p = 0.0001）显著相关。多因素逻辑回归分析表明，肿瘤分化、β-连环蛋白和p53积聚是与CRC中JCV存在显著相关的独立参数（分别为p = 0.04；p = 0.05；p = 0.001）。
我们支持JCV在突尼斯患者结直肠癌发生过程中发挥作用，尤其是在高分化形态的肿瘤中。
