Haghi Navand Azadeh, Teimoori Ali, Makvandi Manoochehr, Nisi Nilofar, Seyedian Seyed Saeid, Ranjbari Nastarn, Ahmadi Angali Kambiz, Keyani Hadis, Tabasi Maryam, Pourjabari Keyvan
Infectious and Tropical Diseases Research Center Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Email: manoochehrmakvandi299@ gmail.com
Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Asian Pac J Cancer Prev. 2019 Apr 29;20(4):1147-1151. doi: 10.31557/APJCP.2019.20.4.1147.
Colorectal cancer is the most repetitious malignancies with high mortality worldwide. JC virus (JCV) is ubiquitous Polyomavirus, with seroprevalence rates ranging from 70% to 90% in adult population. Recently the role of JCV have been reported in many malignant tumors worldwide. The association of JCV was reported in patients with colon and rectum cancers. Thus this study was conducted to evaluate the association of JCV DNA in patients with colon cancer type Adenocarcinoma. Material and Methods: A total of 120 formalin-fixed paraffin-embedded tissue blocks samples were collected including 20/40(50%) males, 20/40(50%) females patients with Colorectal Cancer(CRC), and 80 (50% males, 50% females) patients with benign tumor as a control. DNA was extracted for all the samples. Nested PCR was carried out for detection of Vp1/T-Ag junction genome in JCV genome by Nested-PCR assay. Randomly, PCR products of 6 samples were sequenced to analysis the partial JCV DNA. The phylogeny tree was constructed to determine homology identity with other JCV. Results: 4/40(10%) samples of test group and 10/80 (12.5%) of control samples were positive for JCV DNA (P= 0.69). Out of 4 samples positive for JC DNA, 3(7.5%) were males and 1(2.4%) female (P=0.29). The frequency of JCV DNA in age group> 50 years was 4/32(10%), while in age group <50 years was 0/8 (0%) (p= 0.29). Conclusion: prevalence of JCV DNA was among 10% patients with CRC and 12.5% benign tumors (p=0.69). The distribution of JCV DNA was among 7.5% male and 2.5% female (p= 0.29). The frequency of JCV DNA was among 10% cases of age group >50 years and 0% of age group <50 years (P= 0.29). The subsequent T-Ag protein expression might explain the increased risk of colorectal cancer and requires further investigation.
结直肠癌是全球范围内最常见且死亡率高的恶性肿瘤。JC病毒（JCV）是一种普遍存在的多瘤病毒，在成年人群中的血清阳性率为70%至90%。最近，JCV在全球许多恶性肿瘤中的作用已有报道。JCV与结肠癌和直肠癌患者的关联也有报道。因此，本研究旨在评估JCV DNA在腺癌型结肠癌患者中的关联。材料与方法：共收集120个福尔马林固定石蜡包埋组织块样本，其中包括20/40（50%）男性、20/40（50%）女性的结直肠癌（CRC）患者，以及80名（50%男性，50%女性）患有良性肿瘤的患者作为对照。对所有样本进行DNA提取。通过巢式PCR检测JCV基因组中的Vp1/T-Ag连接基因组。随机选取6个样本的PCR产物进行测序，以分析部分JCV DNA。构建系统发育树以确定与其他JCV的同源性。结果：试验组4/40（10%）样本和对照组10/80（12.5%）样本的JCV DNA呈阳性（P = 0.69）。在4个JCV DNA阳性样本中，3个（7.5%）为男性，1个（2.4%）为女性（P = 0.29）。年龄>50岁组的JCV DNA频率为4/32（10%），而年龄<50岁组为0/8（0%）（p = 0.29）。结论：CRC患者中JCV DNA的患病率为10%，良性肿瘤患者中为12.5%（p = 0.69）。JCV DNA在男性中的分布为7.5%，女性为2.5%（p = 0.29）。JCV DNA在年龄>50岁组的频率为10%，年龄<50岁组为0%（P = 0.29）。随后的T-Ag蛋白表达可能解释了结直肠癌风险的增加，需要进一步研究。
