Toumi Wafa, Ripalti Alessandro, Ricciardiello Luigi, Cherif Abderraouf, Gargouri Dalila, Bouhafa Ahmed, Kharrat Jamel, Jarboui Slim, Benrhouma Hichem, Zili Mohamed, Khelifa Ridha
Viral and Molecular Tumor Diagnostics Unit, Laboratory Services, Habib Thameur Hospital, Tunis, Tunisia.
Microbiology Unit, S. Orsola-Malpighi General Hospital, Bologna, Italy.
New Microbiol. 2017 Apr;40(2):99-106. Epub 2017 Mar 3.
The etiology of colorectal cancer (CRC) remains elusive in spite of major advances in knowledge of this disease and related risk factors. Several studies report the detection of human polyomavirus JC (JCV) in colorectal tumors and some suggest its association with CRC. Since many known human virus associations with cancer are linked to factors such as ethnic and geographical origin, it is interesting to search for the postulated association of JCV with CRC in different populations and regions. In this perspective, the present work was undertaken to assess the presence of JCV in CRC tumors in Tunisia. Fresh biopsies were obtained from both colorectal tumors and adjacent normal tissues of 47 CRC patients. Only tumors diagnosed as adenocarcinomas were included in the present study. Twenty patients with other gastroenterological disorders were taken as controls. DNA was extracted from fresh biopsies or formalin-fixed, paraffin-embedded tissue sections. A region of the viral T-Ag gene was amplified by PCR and the DNA amplicons were subjected to automated sequencing. JCV DNA was found in 22 (46%) of the adenocarcinomas but in none of the normal mucosa biopsies of either CRC or control patients. Sequence analysis indicated that the amplified DNA belonged to a new JCV variant of genotype A. The presence of JCV DNA was correlated with tumor location and grade. The data obtained suggest that JCV may be associated either with a subpopulation of colorectal tumors or with CRC in general, possibly through a hit and run mechanism.
尽管在结直肠癌（CRC）相关知识及危险因素方面取得了重大进展，但其病因仍不明确。多项研究报告在结直肠肿瘤中检测到人类多瘤病毒JC（JCV），一些研究表明其与结直肠癌有关。由于许多已知的人类病毒与癌症的关联与种族和地理起源等因素有关，因此在不同人群和地区探索JCV与结直肠癌的假定关联很有意思。从这个角度出发，本研究旨在评估突尼斯结直肠癌肿瘤中JCV的存在情况。从47例结直肠癌患者的结直肠肿瘤及相邻正常组织中获取新鲜活检样本。本研究仅纳入诊断为腺癌的肿瘤。选取20例患有其他胃肠疾病的患者作为对照。从新鲜活检样本或福尔马林固定、石蜡包埋的组织切片中提取DNA。通过PCR扩增病毒T-Ag基因的一个区域，并对DNA扩增产物进行自动测序。在22例（46%）腺癌中发现了JCV DNA，但在结直肠癌患者或对照患者的正常黏膜活检样本中均未发现。序列分析表明，扩增的DNA属于基因型A的一种新的JCV变体。JCV DNA的存在与肿瘤位置和分级相关。所获得的数据表明，JCV可能与一部分结直肠肿瘤亚群或总体结直肠癌有关，可能是通过一种“打了就跑”的机制。
