Bhosale Shivaji V, Kanhe Nilesh S, Bhoraskar Sudha V, Bhat Suresh K, Bulakhe Ravindra N, Shim Jae-Jin, Mathe Vikas L
Department of Physics, Savitribai Phule Pune University, Pune, 411007, India.
J Mater Sci Mater Med. 2015 Aug;26(8):216. doi: 10.1007/s10856-015-5547-7. Epub 2015 Jul 28.
The paper presents the experimental studies pertaining to the adsorption of bovine serum albumin (BSA) on the nanoparticles of nickel ferrite (NiFe2O4) with a view of correlating the adsorption properties to their microstructure and zeta potentials. Physical properties of two kinds of nickel ferrites, one synthesized by thermal plasma route and the other by chemical co-precipitation method, are compared. Maximum adsorption (231.57 μg/mg) of BSA onto nickel ferrite nanoparticles, at body temperature (37 °C) was observed at pH-value of 5.58 for the thermal plasma synthesized particles showing its higher adsorption capacity than those synthesized by wet chemical means (178.71 μg/mg). Under the same physical conditions the value of zeta potential, obtained for the former, was higher than that of the latter over a wide range of pH values (3.64-9.66). This is attributed to the differences in the specific surface energies of the two kinds of nanoparticles arising from the degree of crystallinity. The paper presents the experimental evidence for the single crystalline nature of the individual nanoparticles, with mean size of 32 nm, for the thermal plasma synthesized particles as evidenced from the high resolution transmission electron microscopy and electron diffraction analysis. The measurements also reveal the poor crystalline morphology in the chemically prepared particles (mean size of 28 nm) although the X-ray diffraction patterns are not much different. The atomic force microscopy images confirm that the surfaces of plasma synthesized nanoparticles possesses higher surface roughness than that of chemically synthesized one. Presence of adsorbed protein was confirmed by vibrational spectroscopy. The Langmuir adsorption model is found to fit into the experimental data better than the Freundlich adsorption model.
本文介绍了有关牛血清白蛋白（BSA）在镍铁氧体（NiFe2O4）纳米颗粒上吸附的实验研究，目的是将吸附特性与其微观结构和zeta电位相关联。比较了两种镍铁氧体的物理性质，一种是通过热等离子体路线合成的，另一种是通过化学共沉淀法合成的。在体温（37°C）下，对于通过热等离子体合成的颗粒，在pH值为5.58时观察到BSA在镍铁氧体纳米颗粒上的最大吸附量（231.57μg/mg），表明其吸附能力高于通过湿化学方法合成的颗粒（178.71μg/mg）。在相同的物理条件下，前者在较宽的pH值范围（3.64 - 9.66）内获得的zeta电位值高于后者。这归因于两种纳米颗粒由于结晶度不同而导致的比表面能差异。本文提供了实验证据，证明通过高分辨率透射电子显微镜和电子衍射分析，热等离子体合成颗粒的单个纳米颗粒平均尺寸为32nm，具有单晶性质。测量还揭示了化学制备颗粒（平均尺寸为28nm）中较差的晶体形态，尽管X射线衍射图谱没有太大差异。原子力显微镜图像证实，等离子体合成纳米颗粒的表面粗糙度高于化学合成的纳米颗粒。通过振动光谱证实了吸附蛋白质的存在。发现Langmuir吸附模型比Freundlich吸附模型更适合实验数据。
