Yin H, Too H P, Chow G M
Department of Materials Science and Engineering, National University of Singapore, Kent Ridge, Singapore 119260, Republic of Singapore.
Biomaterials. 2005 Oct;26(29):5818-26. doi: 10.1016/j.biomaterials.2005.02.036. Epub 2005 Apr 15.
The safety and toxicity of nanoparticles are of growing concern despite their significant scientific interests and promising potentials in many applications. The properties of nanoparticles depend not only on the size but also the structure, microstructure and surface coating. These in turn are controlled by the synthesis and processing conditions. The dependence of cytotoxicity on particle size and on the presence of oleic acid as surfactant on nickel ferrite particles were investigated in vitro using the Neuro-2A cell line as a model. For nickel ferrite particles without oleic acid prepared by ball milling, cytotoxicity was independent of particle size within the given mass concentrations and surface areas accessible to the cells. For nickel ferrite particles coated with oleic acid prepared by the polyol method, the cytotoxicity significantly increased when one or two layers of oleic acid were deposited. Large particles (150+/-50 nm diameter) showed a higher cytotoxicity than smaller particles (10+/-3 nm diameter).
尽管纳米颗粒在许多应用中具有重大的科学意义和广阔的应用前景，但它们的安全性和毒性日益受到关注。纳米颗粒的性质不仅取决于尺寸，还取决于结构、微观结构和表面涂层。而这些又由合成和加工条件所控制。以Neuro-2A细胞系为模型，在体外研究了细胞毒性对镍铁氧体颗粒尺寸以及作为表面活性剂的油酸存在与否的依赖性。对于通过球磨制备的不含油酸的镍铁氧体颗粒，在给定的质量浓度和细胞可接触的表面积范围内，细胞毒性与颗粒尺寸无关。对于通过多元醇法制备的涂有油酸的镍铁氧体颗粒，当沉积一层或两层油酸时，细胞毒性显著增加。大颗粒（直径150±50 nm）比小颗粒（直径10±3 nm）表现出更高的细胞毒性。
