Fletterick C G, Grove T H, Hohnadel D C
Clin Chem. 1979 Mar;25(3):409-12.
We describe a sensitive and precise "high-pressure" liquid-chromatographic method for determining acetaminophen in serum. The 2-acetaminophenol and 3-acetaminophenol structural isomers of acetaminophen are used as internal standards. The method, which involves solvent extraction and adsorption chromatography on silica, provides excellent sensitivity, accuracy, and selectivity. The standard curve is linear over the range of acetaminophen concentrations of 0.5 to 300 mg/L, which makes the method useful for both pharmacokinetic studies and overdose monitoring. Analytical recovery is 97% for acetaminophen concentrations ranging from 5 to 300 mg/L. Many commonly used drugs were tested and found not to interfere. The procedure has been successfully adapted as a microscale method requiring only 50 microL of sample. The microscale method is particularly useful for pediatric and neonatal patients for whom sample size is a major concern.
我们描述了一种灵敏且精确的“高压”液相色谱法，用于测定血清中的对乙酰氨基酚。对乙酰氨基酚的2-乙酰氨基酚和3-乙酰氨基酚结构异构体用作内标。该方法包括溶剂萃取和硅胶吸附色谱法，具有出色的灵敏度、准确性和选择性。在对乙酰氨基酚浓度为0.5至300 mg/L的范围内，标准曲线呈线性，这使得该方法可用于药代动力学研究和过量用药监测。对乙酰氨基酚浓度在5至300 mg/L范围内时，分析回收率为97%。对许多常用药物进行了测试，发现它们不会产生干扰。该方法已成功改编为仅需50微升样品的微量方法。这种微量方法对于样本量是主要关注点的儿科和新生儿患者特别有用。
