Pinto Sandra Cristina Girotto, Bueno Fernanda Giacomini, Panizzon Gean Pier, Morais Gutierrez, Dos Santos Paulo Victor Pires, Baesso Mauro Luciano, Leite-Mello Eneri Vieira de Souza, de Mello João Carlos Palazzo
Departamento de Farmácia, Laboratório de Biologia Farmacêutica, Palafito, Universidade Estadual de Maringá, Maringá, Brazil.
Departamento de Física, Grupo de Fenômenos Fototérmicos, Universidade Estadual de Maringá, Maringá, Brazil.
Planta Med. 2015 Aug;81(12-13):1090-6. doi: 10.1055/s-0035-1546209. Epub 2015 Jul 28.
Diabetes mellitus is a serious public health problem in which a major complication is impaired wound healing. Among the strategies developed to foster tissue repair is the use of medicinal plants. The bark of Stryphnodendron adstringens, which is popularly used as an aid in wound healing, has a documented effect on wound repair in normal rats. This study evaluated the healing action of the crude extract of S. adstringens in diabetic rats, and its chemical content. Compounds present in the crude extract were characterized by mass spectrometry. In diabetic rats (streptozotocin 35 mg/kg), two wounds made in the skin were treated daily for 4, 7, 10, and 14 days with gel containing 1 % crude extract or with base gel. Histological analyses involved the measurement of the length and thickness of the re-epithelialized surface, quantification of the number of cells in mitosis, and types I and III collagen fibers. Also, cutaneous permeation by photoacoustic spectroscopy, and the expression of cyclooxygenase-2 and vascular endothelial growth factor by Western blot were assessed. The crude extract fingerprint showed masses indicating proanthocyanidins. The crude extract mainly stimulated cell migration and proliferation of keratinocytes at the beginning of the treatment in addition to stimulating the replacement of type III collagen fibers by type I collagen fibers at 10 and 14 days. The photoacoustic spectroscopy technique showed that the gel containing 1 % of crude extract permeated through the skin to the dermis, where the crude extract was found. Vascular endothelial growth factor was stimulated after 7 days of treatment with the crude extract and cyclooxygenase-2 at 4, 7, and 10 days. The crude extract of S. adstringens acted in tissue repair in wounds in diabetic rats by stimulating the production of collagen fibers at the wound site. The crude extract favored the formation of a more organized extracellular matrix and filled the entire extent of the wound, and also fostered the upregulation of cyclooxygenase-2 and vascular endothelial growth factor, which are essential to this process. These crude extract actions in diabetic wounds are probably due to the presence of proanthocyanidins.
糖尿病是一个严重的公共卫生问题，其主要并发症之一是伤口愈合受损。在为促进组织修复而开发的策略中，使用药用植物是其中之一。人们普遍使用收敛鼠李（Stryphnodendron adstringens）的树皮来辅助伤口愈合，该树皮对正常大鼠的伤口修复有显著效果。本研究评估了收敛鼠李粗提物对糖尿病大鼠伤口的愈合作用及其化学成分。通过质谱对粗提物中的化合物进行了表征。在糖尿病大鼠（链脲佐菌素35 mg/kg）身上，在皮肤上制造两个伤口，每天用含1%粗提物的凝胶或基础凝胶处理4、7、10和14天。组织学分析包括测量再上皮化表面的长度和厚度、有丝分裂细胞数量的定量以及I型和III型胶原纤维的类型。此外，通过光声光谱法评估皮肤渗透情况，并通过蛋白质印迹法评估环氧合酶-2和血管内皮生长因子的表达。粗提物指纹图谱显示有表明原花青素的质谱峰。粗提物在治疗开始时主要刺激角质形成细胞的迁移和增殖，此外在第10天和第14天刺激I型胶原纤维取代III型胶原纤维。光声光谱技术表明，含1%粗提物的凝胶可透过皮肤到达真皮，在真皮中发现了粗提物。用粗提物处理7天后刺激了血管内皮生长因子的产生，在第4、7和10天刺激了环氧合酶-2的产生。收敛鼠李粗提物通过刺激糖尿病大鼠伤口部位胶原纤维的产生，对伤口组织修复起作用。粗提物有利于形成更有组织的细胞外基质并填充整个伤口范围，还促进了环氧合酶-2和血管内皮生长因子的上调，而这两者对该过程至关重要。这些粗提物在糖尿病伤口中的作用可能归因于原花青素的存在。
