Salazar Gerson Javier Torres, Dias Francisco Junio, Ribeiro Paulo Riceli Vasconcelos, de Brito Edy Sousa, Canuto Kirley Marques, Coutinho Henrique Douglas Melo, Ribeiro-Filho Jaime, Gallo Monica, Montesano Domenico, Naviglio Daniele, Zengin Gokhan, da Costa José Galberto Martins
Postgraduate Program in Ethnobiology and Nature Conservation, Regional University of Cariri, Coronel Antônio Luíz Street, 1161-Pimenta, Crato 63105-010, Brazil.
Multi-User Laboratory of Natural Products Chemistry, Embrapa Tropical Agroindustry, Sara Mesquita, no 2.270, Neighborhood Planalto do Pici, Fortaleza 60511-110, Brazil.
Foods. 2021 Nov 3;10(11):2683. doi: 10.3390/foods10112683.
Mart., popularly known as "barbatimão", is a plant species traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases. Considering the well-described antioxidant properties of this species, this study investigated the protective effects of its keto-aqueous extract using an in vitro model of iron overload. Phenolic compounds were quantified and identified by Ultra-Performance Liquid Chromatography coupled with quadrupole Time-Of-Flight Electrospray Ionization Mass Spectrometry () in positive and negative ions mode analysis. Antioxidant activity was analyzed following the iron-chelating-reducing capacity and deoxyribose degradation (2-DR) protection methods. The analysis identified condensed tannins (54.8 mg catechin/g dry fraction (DF), polyphenols (25 mg gallic acid/g DF), and hydrolyzable tannins (28.8 mg tannic acid/g DF). Among the constituents, prodelphinidin, procyanidin, and prorobinetinidine were isolated and identified. The extract significantly protected 2-DR degradation induced by Fe (72% protection) or OH (43% protection). The -phenanthroline test revealed Fe-chelating and Fe-reducing activities of 93% and 84%, respectively. A preliminary toxicological analysis using revealed mortality below 10%, at a concentration of 0.25 mg/mL, indicating low toxicity under the present experimental conditions. In conclusion, the findings of the present study indicate that is a source of antioxidant compounds with the potential to be used in drug development in the context of iron overload disorders, which remains to be further investigated in vivo.
马丁（Mart.），俗称“巴巴蒂芒”（barbatimão），是一种传统上通过局部和口服途径用于治疗感染性和炎症性疾病的植物物种。鉴于该物种已被充分描述的抗氧化特性，本研究使用铁过载的体外模型研究了其酮水提取物的保护作用。通过超高效液相色谱结合四极杆飞行时间电喷雾电离质谱（）在正离子和负离子模式分析下对酚类化合物进行定量和鉴定。采用铁螯合还原能力和脱氧核糖降解（2-DR）保护方法分析抗氧化活性。分析鉴定出缩合单宁（54.8毫克儿茶素/克干馏分（DF））、多酚（25毫克没食子酸/克DF）和可水解单宁（28.8毫克单宁酸/克DF）。在这些成分中，原花青定、原花青素和原刺槐素被分离和鉴定。该提取物显著保护了由Fe（72%保护率）或OH（43%保护率）诱导的2-DR降解。邻菲罗啉试验显示铁螯合和铁还原活性分别为93%和84%。使用进行的初步毒理学分析表明，在浓度为0.25毫克/毫升时死亡率低于10%，表明在当前实验条件下毒性较低。总之，本研究结果表明，马丁是抗氧化化合物的一个来源，在铁过载疾病的背景下具有用于药物开发的潜力，这仍有待在体内进一步研究。
