De Leenheer A P, De Bevere V O, Claeys A E
Clin Chem. 1979 Mar;25(3):425-8.
A liquid-chromatographic assay for alpha, beta, and gamma-isomeric tocopherols in human serum is reported. The tocopherols and the internal standard (tocol) are absorbed into a silica gel column and are eluted in less than 10 min with n-hexane/isopropanol (99.4/0.6, by vol) at a flow rate of 1 mL/min. The complete analysis requires no longer than 30 min. Within-day precision (CV) was 1.4% (mean = 13.18 mg/L, n = 24), 7.4% (mean = 0.214 mg/L, n = 14), and 1.3% (mean = 1.01 mg/L, n = 14) for alpha-, beta-, and gamma-tocopherol, respectively. Day-to-day precision (CV) was 4.4% (mean = 9.85 mg/L, n = 10) for alpha-tocopherol, 9.1% (mean = 0.222 mg/L, n = 20) for beta-tocopherol, and 3.8% (mean = 1.00 mg/L, n = 20) for gamma-tocopherol. Extraction recoveries for alpha-, beta-, and gamma-tocopherol averaged 92.4 +/- 2.9% (n = 5), 91.4 4/- 7.6% (n = 5), an 92.0 +/- 4.1% (n = 4), respectively. The smallest injected amount detectable is estimated to be 0.03 micrograms for alpha-, and 0.04 micrograms for beta- and gamma-tocopherol.
本文报道了一种用于测定人血清中α、β和γ异构体生育酚的液相色谱分析法。生育酚和内标（tocol）被吸附到硅胶柱上，以1 mL/min的流速用正己烷/异丙醇（体积比99.4/0.6）在不到10分钟内洗脱。完整分析所需时间不超过30分钟。日内精密度（CV）对于α-生育酚为1.4%（平均值 = 13.18 mg/L，n = 24），对于β-生育酚为7.4%（平均值 = 0.214 mg/L，n = 14），对于γ-生育酚为1.3%（平均值 = 1.01 mg/L，n = 14）。日间精密度（CV）对于α-生育酚为4.4%（平均值 = 9.85 mg/L，n = 10），对于β-生育酚为9.1%（平均值 = 0.222 mg/L，n = 20），对于γ-生育酚为3.8%（平均值 = 1.00 mg/L，n = 20）。α-、β-和γ-生育酚的提取回收率平均分别为92.4±2.9%（n = 5）、91.4±7.6%（n = 5）和92.0±4.1%（n = 4）。估计可检测到的最小进样量对于α-生育酚为0.03微克，对于β-和γ-生育酚为0.04微克。
