Department of Electronic Engineering, City University of Hong Kong, Kowloon, Hong Kong; Center for Biosystems, Neuroscience and Nanotechnology, City University of Hong Kong, Kowloon, Hong Kong; Institute of Electronic Engineering, China Academy of Engineering Physics, Mianyang, China.
Center for Biosystems, Neuroscience and Nanotechnology, City University of Hong Kong, Kowloon, Hong Kong; Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong.
Biosens Bioelectron. 2015 Dec 15;74:799-807. doi: 10.1016/j.bios.2015.07.022. Epub 2015 Jul 11.
The double resonance plasmonic biosensors based on Au nanodisks (NDs) with a thin SiO2 spacer between the top and bottom Au layers were employed to detect MCF-7 breast cancer cells. The hybridized modes between the localized surface plasmon resonance of Au NDs and the gap coupling resonance of NDs with the Au film underneath have been observed. These multiple metallic layer NDs exhibit higher sensitivity than the common single metallic layer NDs. The extinction spectra showed double resonance bands that could be tailored by varying the ND size. Three sizes of multiple layer NDs ranging from 60 to 200 nm diameter (dia.) were generated and their refractive sensitivity to the surrounding media were analyzed for cell detection. Nanodisks with 120 dia. showed the highest refractive sensitivity up to 230 nm/refractive index unit. These sensors could be used to detect a broad range of MCF-7 cells from a low cell concentration down of 1.0×10(3)cells/ml up to a high cell concentration of 1.7×10(7) cells/ml.
基于金纳米盘（AuNDs）的双共振等离子体生物传感器，其上下 Au 层之间有一个薄的 SiO2 间隔物，用于检测 MCF-7 乳腺癌细胞。已经观察到 AuNDs 的局域表面等离子体共振与 Nd 与下方 Au 膜之间的间隙耦合共振之间的杂化模式。这些多层 Nd 表现出比常见的单层 Nd 更高的灵敏度。消光谱显示出双共振带，通过改变 Nd 的尺寸可以对其进行调整。生成了三种尺寸的从 60 到 200nm 直径（Dia.）的多层 Nd，并分析了它们对周围介质的折射率灵敏度以用于细胞检测。直径为 120nm 的 Nd 表现出高达 230nm/折射率单位的最高折射率灵敏度。这些传感器可用于检测低浓度（低至 1.0×10(3)个细胞/ml）至高浓度（高达 1.7×10(7)个细胞/ml）的 MCF-7 细胞。
