Moore Simon, Zhang Xiaoxian, Liu Junli, Lindsey Keith
a The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, Durham University ; Durham , UK.
b School of Engineering, The University of Liverpool ; Liverpool , UK.
Plant Signal Behav. 2015;10(10):e1056424. doi: 10.1080/15592324.2015.1056424. Epub 2015 Aug 3.
The activities of hormones in the Arabidopsis root depend on cellular context and exhibit either synergistic or antagonistic interactions. Patterning in Arabidopsis root development is coordinated via a localized auxin concentration maximum in the root tip, mediating transcription of key regulatory genes. Auxin concentration and response are each regulated by diverse interacting hormones and gene expression and therefore cannot change independently of those hormones and genes. For example, experimental data accumulated over many years have shown that both ethylene and cytokinin regulate auxin concentration and response. Using the crosstalk of auxin-ethylene-cytokinin as a paradigm, we discuss the links between experimental data, reaction kinetics and spatiotemporal modeling to dissect hormonal crosstalk. In particular, we discuss how kinetic equations for modeling auxin concentration are formulated based on experimental data and also the underlying assumptions for deriving those kinetic equations. Furthermore, we show that, by integrating kinetic equations with spatial root structure, modeling of spatiotemporal hormonal crosstalk is a powerful tool for analyzing and predicting the roles of multiple hormone interactions in auxin patterning. Finally, we summarize important considerations in developing a spatiotemporal hormonal crosstalk model for plant root development.
激素在拟南芥根中的活性取决于细胞环境，并表现出协同或拮抗相互作用。拟南芥根发育过程中的模式形成是通过根尖局部生长素浓度最大值来协调的，该最大值介导关键调控基因的转录。生长素浓度和反应分别受多种相互作用的激素以及基因表达的调控，因此不能独立于这些激素和基因而发生变化。例如，多年积累的实验数据表明，乙烯和细胞分裂素都能调节生长素浓度和反应。以生长素 - 乙烯 - 细胞分裂素的相互作用为范例，我们讨论实验数据、反应动力学和时空建模之间的联系，以剖析激素间的相互作用。特别是，我们讨论如何根据实验数据制定生长素浓度建模的动力学方程，以及推导这些动力学方程的潜在假设。此外，我们表明，通过将动力学方程与根的空间结构相结合，时空激素相互作用建模是分析和预测多种激素相互作用在生长素模式形成中作用的有力工具。最后，我们总结了为植物根发育建立时空激素相互作用模型时的重要考虑因素。
