The Integrative Cell Biology Laboratory and The Biophysical Sciences Institute, School of Biological and Biomedical Sciences, Durham University, Durham, UK.
Mol Syst Biol. 2010 Jun 8;6:373. doi: 10.1038/msb.2010.26.
An important question in plant biology is how genes influence the crosstalk between hormones to regulate growth. In this study, we model POLARIS (PLS) gene function and crosstalk between auxin, ethylene and cytokinin in Arabidopsis. Experimental evidence suggests that PLS acts on or close to the ethylene receptor ETR1, and a mathematical model describing possible PLS-ethylene pathway interactions is developed, and used to make quantitative predictions about PLS-hormone interactions. Modelling correctly predicts experimental results for the effect of the pls gene mutation on endogenous cytokinin concentration. Modelling also reveals a role for PLS in auxin biosynthesis in addition to a role in auxin transport. The model reproduces available mutants, and with new experimental data provides new insights into how PLS regulates auxin concentration, by controlling the relative contribution of auxin transport and biosynthesis and by integrating auxin, ethylene and cytokinin signalling. Modelling further reveals that a bell-shaped dose-response relationship between endogenous auxin and root length is established via PLS. This combined modelling and experimental analysis provides new insights into the integration of hormonal signals in plants.
植物生物学中的一个重要问题是基因如何影响激素之间的串扰，以调节生长。在这项研究中，我们对 POLARIS（PLS）基因的功能以及拟南芥中生长素、乙烯和细胞分裂素之间的串扰进行了建模。实验证据表明，PLS 作用于或靠近乙烯受体 ETR1，并且开发了一个描述可能的 PLS-乙烯途径相互作用的数学模型，并用于对 PLS-激素相互作用进行定量预测。该模型正确地预测了 pls 基因突变对细胞分裂素内源性浓度的影响的实验结果。该模型还揭示了 PLS 在生长素生物合成中的作用，除了在生长素运输中的作用之外。该模型再现了现有的突变体，并结合新的实验数据，通过控制生长素运输和生物合成的相对贡献以及整合生长素、乙烯和细胞分裂素信号，提供了关于 PLS 如何调节生长素浓度的新见解。该模型进一步表明，通过 PLS 建立了内源生长素和根长之间的钟形剂量反应关系。这种组合建模和实验分析为植物中激素信号的整合提供了新的见解。
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