Krishnamurithy Genasan, Murali Malliga Raman, Hamdi Mohd, Abbas Azlina Amir, Raghavendran Hanumantharao Balaji, Kamarul Tunku
Tissue Engineering Group (TEG), Department of Orthopaedic Surgery, NOCERAL, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Institute of Translational Medicine, University of Liverpool, Liverpool L69 3GE, UK.
Regen Med. 2015;10(5):579-90. doi: 10.2217/rme.15.27.
To compare the effect of bovine bone derived porous hydroxyapatite (BDHA) scaffold on proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells (hMSCs) compared with commercial hydroxyapatite (CHA) scaffold.
The porosity and pore size were analyzed using micro-CT. The biocompatibility was demonstrated by alamar blue assay, and cell attachment through SEM and Hoechst staining. The osteogenic differentiation was demonstrated using biochemical assay and osteogenic gene expression.
BDHA and CHA scaffolds showed porosity of 76.6 ± 0.6 and 64.3 ± 0.3% and pore size diameter of 0.04-0.25 and 0.1-2.6 mm, respectively. hMSCs proliferation, ALP activity, osteocalcin secretion and osteogenic gene expression are comparable in both the scaffolds.
These results demonstrated that BDHA is biocompatible, supports cell adhesion and promotes proliferation and osteogenic differentiation.
比较牛骨衍生多孔羟基磷灰石（BDHA）支架与人骨髓间充质基质细胞（hMSCs）增殖和成骨分化的效果，并与商用羟基磷灰石（CHA）支架进行对比。
使用微型计算机断层扫描（micro-CT）分析孔隙率和孔径。通过alamar蓝分析法证明生物相容性，并通过扫描电子显微镜（SEM）和Hoechst染色观察细胞附着情况。使用生化分析法和成骨基因表达证明成骨分化情况。
BDHA和CHA支架的孔隙率分别为76.6±0.6%和64.3±0.3%，孔径分别为0.04 - 0.25毫米和0.1 - 2.6毫米。两种支架中hMSCs的增殖、碱性磷酸酶（ALP）活性、骨钙素分泌和成骨基因表达相当。
这些结果表明BDHA具有生物相容性，支持细胞黏附，并促进增殖和成骨分化。
