Zhan Gang-ming, Wang Fu-ping, Luo Huai-yong, Jiang Shu-chang, Zheng Wen-ming, Huang Li-li, Kang Zhen-sheng
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China.
State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Life Science, Henan Agricultural University, Zhengzhou 450002, China.
J Zhejiang Univ Sci B. 2015 Aug;16(8):727-32. doi: 10.1631/jzus.B1400364.
Puccinia striiformis f. sp. tritici (Pst) is the obligate biotrophic fungus responsible for stripe rust wheat. In this study, we developed and characterized 20 polymorphic microsatellite markers from the genomic sequence of an isolate of Chinese Pst race CY32. Polymorphism at each simple sequence repeat (SSR) locus was determined using 32 Pst isolates from 7 countries. The number of alleles varied from 2 to 7 across isolates, and the observed and expected heterozygosities ranged from 0.33 to 0.97 (mean 0.62) and 0.23 to 0.73 (mean 0.51), respectively. As expected the genomic SSR markers were more polymorphic than the expressed sequence tag (EST)-SSR markers developed previously. These markers will be more useful for population genetics and molecular genetics studies in Pst.
条形柄锈菌小麦专化型（Pst）是导致小麦条锈病的专性活体营养型真菌。在本研究中，我们从中国Pst小种CY32一个分离株的基因组序列开发并鉴定了20个多态性微卫星标记。使用来自7个国家的32个Pst分离株确定了每个简单序列重复（SSR）位点的多态性。各分离株的等位基因数从2到7不等，观察到的杂合度和预期杂合度分别为0.33至0.97（平均0.62）和0.23至0.73（平均0.51）。正如预期的那样，基因组SSR标记比之前开发的表达序列标签（EST）-SSR标记具有更高的多态性。这些标记将对Pst的群体遗传学和分子遗传学研究更有用。
