Chen Wen, Zhang Zedong, Tian Ye, Kang Zhensheng, Zhao Jie
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100 China.
Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang, 550006 China.
J Plant Pathol. 2023;105(1):253-267. doi: 10.1007/s42161-022-01273-1. Epub 2022 Dec 21.
Wheat stripe rust, caused by f. sp. (), is an extremely destructive wheat disease worldwide. Yunnan and Guizhou provinces are the main overwintering regions for and inoculum sources for the disease in China. Surveillance of race dynamics of the population is essential for managing the disease in local and other wheat-growing regions of China. However, population dynamics in this region is not monitored yearly. In this study, a population of 113 isolates from Yunnan and Guizhou in 2018 were phenotyped on the two wheat differential sets and analyzed by 12 simple sequence repeat (SSR) markers. As a result, 25 races were identified from the population on Chinese differentials and predominant races was CYR32 with a frequency of 17.70%. None of the isolates was virulent to and Only a few isolates showed virulence to and differentials Zhong 4. The Kosman index () of the population was 0.292 on the Chinese differentials, and 0.274 on single gene lines and additional differentials, respectively. In total, 64 MLGs were detected among 113 isolates and the expected heterozygosity () was 0.424. A close genetic relationship was detected between Lufeng (Yunnan) / Nayong (Guizhou), Fuyuan (Yunnan) / Bijie (Guizhou), and Shizong (Yunnan) / Xingyi (Guizhou). This study provided useful information on population structure and virulence to genes in the Yunnan and Guizhou epidemiological regions, and will be used to guide the control of wheat stripe rust and targeted wheat breeding in this region.
The online version contains supplementary material available at 10.1007/s42161-022-01273-1.
由条形柄锈菌小麦专化型（Puccinia striiformis f. sp. tritici）引起的小麦条锈病是一种在全球范围内极具破坏性的小麦病害。云南省和贵州省是中国小麦条锈病菌的主要越冬区和病菌接种源地。监测该病菌群体的生理小种动态对于中国当地及其他小麦种植区的病害管理至关重要。然而，该地区病菌群体动态并非每年都进行监测。在本研究中，对2018年来自云南和贵州的11３个病菌分离株群体在两套小麦鉴别寄主上进行了表型分析，并通过12个简单序列重复（SSR）标记进行了分析。结果显示，在中国鉴别寄主上从该病菌群体中鉴定出25个生理小种，优势生理小种为CYR32，频率为17.70%。没有分离株对Yr1和Yr26有毒性。只有少数分离株对Yr5和鉴别寄主中4有毒性。该病菌群体在中国鉴别寄主上的科斯曼指数（Kosman index）为0.292，在单个Yr基因系和附加鉴别寄主上分别为0.274。在113个分离株中总共检测到64个多基因谱系（MLGs），预期杂合度（He）为0.424。在禄丰（云南）/纳雍（贵州）、富源（云南）/毕节（贵州）和师宗（云南）/兴义（贵州）之间检测到密切的遗传关系。本研究提供了关于云南和贵州流行区病菌群体结构及对Yr基因毒性的有用信息，并将用于指导该地区小麦条锈病的防治和有针对性的小麦育种。
在线版本包含可在10.1007/s42161-022-01273-1获取的补充材料。
