Zou Yingxin, Liu Yuxiang, Ruan Minghua, Feng Xu, Wang Jiachun, Chu Zhiyong, Zhang Zesheng
Naval Medical Research Institute, Shanghai 200433, P.R. China.
Department of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
Int J Mol Med. 2015 Oct;36(4):939-46. doi: 10.3892/ijmm.2015.2296. Epub 2015 Jul 30.
This study investigated the effect of Cordyceps sinensis oral liquid (CSOL) on the lifespan of Drosophila melanogaster (fruit fly). Following the lifelong treatment of fruit flies with CSOL, lifespan was examined. The activity of copper-zinc-containing superoxide dismutase 1 (SOD1), manganese-containing superoxide dismutase 2 (SOD2) and catalase (CAT), as well as the lipofuscin (LF) content were determined. The mRNA levels of SOD1, SOD2 and CAT were quantified by qPCR. Hydrogen peroxide (H2O2) and paraquat were used to mimic the effects of damage caused by acute oxidative stress. D-galactose was used to mimic chronic pathological aging. CSOL significantly prolonged the lifespan of the fruit flies under physiological conditions. The activity of SOD1 and CAT was upregulated, and LF accumulation was inhibited by CSOL. CSOL had no effect on the transcriptional levels (mRNA) of these enzymes. The survival time of the fruit flies which were negatively affected by exposure to H2O2 or paraquat was significantly prolonged by CSOL. In fruit flies pathologically aged by epxosure to D-galactose, CSOL also significantly prolonged their lifespan, upregulated the activity of SOD1 and CAT, and inhibited LF accumulation. The findings of our study indicate that CSOL prolongs the lifespan of fruit flies through an anti-oxidative stress pathway involving the upregulation of SOD1 and CAT activity and the inhibition of LF accumulation. CSOL may thus be explored as a novel agent for slowing the human aging process.
本研究调查了中华虫草口服液（CSOL）对黑腹果蝇（果蝇）寿命的影响。在用CSOL对果蝇进行终身处理后，检测其寿命。测定了含铜锌超氧化物歧化酶1（SOD1）、含锰超氧化物歧化酶2（SOD2）和过氧化氢酶（CAT）的活性以及脂褐素（LF）含量。通过qPCR定量SOD1、SOD2和CAT的mRNA水平。用过氧化氢（H2O2）和百草枯模拟急性氧化应激造成的损伤效应。用D-半乳糖模拟慢性病理衰老。CSOL在生理条件下显著延长了果蝇的寿命。CSOL上调了SOD1和CAT的活性，并抑制了LF积累。CSOL对这些酶的转录水平（mRNA）没有影响。CSOL显著延长了受到H2O2或百草枯负面影响的果蝇的存活时间。在因暴露于D-半乳糖而发生病理衰老的果蝇中，CSOL也显著延长了它们的寿命，上调了SOD1和CAT的活性，并抑制了LF积累。我们的研究结果表明，CSOL通过上调SOD1和CAT活性以及抑制LF积累的抗氧化应激途径延长了果蝇的寿命。因此，CSOL可作为一种延缓人类衰老进程的新型药物进行探索。
