Zou Y-X, Ruan M-H, Luan J, Feng X, Chen S, Chu Z-Y
Zhi-Yong Chu, Naval Medical Research Institute, 880 Xiangyin Road, Shanghai, China. Tel: +86(21)81883188,
J Nutr Health Aging. 2017;21(3):314-319. doi: 10.1007/s12603-016-0752-8.
This study investigated the effect of riboflavin on aging in Drosophila melanogaster (fruit fly).
Experimental study.
Naval Medical Research Institute.
Fruit fly Drosophila melanogaster.
After lifelong supplement of riboflavin, the lifespan and the reproduction of fruit flies were observed. Hydrogen peroxide (H2O2) was used to mimic oxidative stress damage to fruit flies and the survival time was recorded.
The activity of copper-zinc-containing superoxide dismutase (SOD1), manganese containing SOD (SOD2) and catalase (CAT) and lipofuscin (LF) content were determined.
Riboflavin significantly prolonged the lifespan (Log rank χ2=16.677, P<0.001) and increased the reproductive capacity (P<0.01 for day 15; P<0.05 for day 30) of fruit flies by lifelong supplement. The survival time of fruit flies damaged by H2O2 was significantly prolonged (Log rank χ2=15.886, P<0.001), the activity of SOD1 (P<0.01) and CAT (P<0.01) was enhanced, and the accumulation of LF (P<0.01) was inhibited by riboflavin supplement.
Riboflavin prolonged the lifespan and increased the reproduction of fruit flies through anti-oxidative stress pathway involving enhancing the activity of SOD1 and CAT and inhibiting LF accumulation. Riboflavin deserves more attention for slowing human aging.
本研究调查了核黄素对黑腹果蝇衰老的影响。
实验研究。
海军医学研究所。
黑腹果蝇。
在果蝇终生补充核黄素后，观察其寿命和繁殖情况。用过氧化氢（H2O2）模拟果蝇的氧化应激损伤并记录存活时间。
测定含铜锌超氧化物歧化酶（SOD1）、含锰超氧化物歧化酶（SOD2）和过氧化氢酶（CAT）的活性以及脂褐素（LF）含量。
终生补充核黄素可显著延长果蝇的寿命（对数秩检验χ2=16.677，P<0.001）并提高其繁殖能力（第15天P<0.01；第30天P<0.05）。核黄素补充可显著延长H2O2损伤果蝇的存活时间（对数秩检验χ2=15.886，P<0.001），增强SOD1（P<0.01）和CAT（P<0.01）的活性，并抑制LF的积累（P<0.01）。
核黄素通过增强SOD1和CAT的活性以及抑制LF积累的抗氧化应激途径延长了果蝇的寿命并提高了其繁殖能力。核黄素在延缓人类衰老方面值得更多关注。
