Vardevanyan Poghos O, Antonyan Ara P, Parsadanyan Marine A, Torosyan Margarita A, Karapetian Armen T
a Department of Biophysics , Yerevan State University , Alex Manoogian 1, 0025 Yerevan , Armenia.
b Department of Physics and Electric Engineering , National University of Architecture and Construction , Yerevan 0009 , Armenia.
J Biomol Struct Dyn. 2016 Jul;34(7):1377-82. doi: 10.1080/07391102.2015.1079557. Epub 2016 Apr 18.
Large amount of data of experimental and theoretical studies have shown that ethidium bromide (EtBr) and methylene blue (MB) may bind to nucleic acids via three modes: intercalation between two adjacent base pairs, insertion into the plane between neighboring bases in the same strand (semi-intercalation), and outside binding with negatively charged backbone phosphate groups. The aim of the given research is to examine the behavior of these two ligands at both separate and joint DNA binding. The obtained experimental data show that the effect of simultaneous binding of EtBr and MB on double-stranded DNA has a non-additive effect of separate binding. The analyses of the melting thermodynamic parameters of DNA complexes with two bound ligands suggest competitive mechanism of interaction.
大量的实验和理论研究数据表明，溴化乙锭（EtBr）和亚甲蓝（MB）可通过三种模式与核酸结合：插入两个相邻碱基对之间、插入同一条链中相邻碱基之间的平面（半插入）以及与带负电荷的主链磷酸基团进行外部结合。本研究的目的是考察这两种配体在单独和联合与DNA结合时的行为。所获得的实验数据表明，EtBr和MB同时结合对双链DNA的影响具有单独结合的非加和效应。对含有两种结合配体的DNA复合物的解链热力学参数分析表明存在相互作用的竞争机制。
