Jian Li, Xin Lin, Yufang Ma, Yifan Huang
University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China.
Pharmacogn Mag. 2015 Jul-Sep;11(43):524-32. doi: 10.4103/0973-1296.160461.
Calycosin-7-O-β-D-glucopyranoside (CG) is a natural isoflavone found in traditional Chinese medicines Astragali Radix (AR).
Calycosin-7-O-β-D-glucopyranoside, an isoflavone isolated from AR, has been found to have potent antioxidantive effects. This study was designed to investigate whether CG prevents oxidative stress induced by thioacetamide (TAA).
BRL-3A cells were pretreated with different concentrations of CG (10, 20, 40 mg/mL) for 12 h and then exposed to 0.18 mol/L TAA for 2 h. The cell viability were examined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium assay, total antioxidant capacity, malondialdehyde (MDA) and the activity of antioxidant enzymes, including catalase, glutathione peroxidase and superoxide dismutase were determined by microplate method. Reactive oxygen species (ROS) generation was quantified by the 2',7'-dichlorofluorescin-diacetate method. Protein and mRNA expression of CYP2E1 were determined by western blotting and real-time PCR.
The cell oxidative stress was significantly increased after 2 h of TAA exposure. Pretreatment of BRL-3A cells with CG significantly increased the activities of antioxidant enzymes, scavenged ROS and reduced MDA production. CG decreased the expression of CYP2E1, and ultimately decreased TAA-induced BRL-3A cells oxidative stress.
Calycosin-7-O-β-D-glucopyranoside has a protective effect against TAA-induced oxidative stress in BRL-3A cells, and that the underlying mechanism involves in scavenging of ROS and the modulating expression of CYP2E1.
毛蕊异黄酮葡萄糖苷（CG）是一种存在于传统中药黄芪中的天然异黄酮。
从黄芪中分离得到的异黄酮毛蕊异黄酮葡萄糖苷已被发现具有强大的抗氧化作用。本研究旨在探讨CG是否能预防硫代乙酰胺（TAA）诱导的氧化应激。
用不同浓度的CG（10、20、40mg/mL）预处理BRL-3A细胞12小时，然后暴露于0.18mol/L TAA中2小时。采用3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑盐法检测细胞活力，用微孔板法测定总抗氧化能力、丙二醛（MDA）以及抗氧化酶（包括过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶）的活性。采用2',7'-二氯荧光素二乙酸酯法对活性氧（ROS）生成进行定量。通过蛋白质印迹法和实时聚合酶链反应测定CYP2E1的蛋白质和mRNA表达。
TAA暴露2小时后，细胞氧化应激显著增加。用CG预处理BRL-3A细胞可显著提高抗氧化酶活性，清除ROS并减少MDA生成。CG降低了CYP2E1表达，最终降低了TAA诱导的BRL-3A细胞氧化应激。
毛蕊异黄酮葡萄糖苷对TAA诱导的BRL-3A细胞氧化应激具有保护作用，其潜在机制涉及ROS的清除和CYP2E1表达的调节。
