Seo Chang-Seob, Shin Hyeun-Kyoo
K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
Pharmacogn Mag. 2015 Jul-Sep;11(43):562-6. doi: 10.4103/0973-1296.160471.
Aucklandia lappa Decne, a well-known traditional herbal medicine, is used for the treatment of asthma, rheumatism, coughs, tuberculosis, and many other diseases.
We performed simultaneous analysis of three sesquiterpene lactones, costunolide (1), dehydrocostus lactone (2), and alantolactone (3), obtained from a 70% methanol extract of A. lappa using high-performance liquid chromatography-photodiode array (HPLC-PDA) techniques.
The compounds 1-3 were separated using a reversed-phase SunFire™ C18 analytical column kept at 35°C by the isocratic elution with distilled water and acetonitrile as mobile phase. The flow rate was 1.0 mL/min, and the injection volume was 10 μL.
The established analytical method showed high linearity, with a correlation coefficient ≥0.9999. The limit of detection and the limit of quantification of compounds 1-3 were 0.06-0.13 μg/mL and 0.21-0.42 μg/mL, respectively. The recovery of the compounds 1-3 was 97.27-103.00%. The intra- and inter-day relative standard deviations were 0.09-0.97% and 0.09-1.06%, respectively. The amounts of the compounds 1-3 were 17.32, 28.26, and 0.01 mg/g, respectively.
The established and validated HPLC-PDA method may be help for the quality control of herbal medicine, A. lappa.
木香是一种著名的传统草药，用于治疗哮喘、风湿病、咳嗽、肺结核和许多其他疾病。
我们使用高效液相色谱-光电二极管阵列（HPLC-PDA）技术，对从木香70%甲醇提取物中获得的三种倍半萜内酯——木香内酯（1）、去氢木香内酯（2）和阿兰内酯（3）进行了同时分析。
化合物1-3使用反相SunFire™ C18分析柱进行分离，柱温保持在35°C，以蒸馏水和乙腈作为流动相进行等度洗脱。流速为1.0 mL/min，进样量为10 μL。
所建立的分析方法显示出高线性，相关系数≥0.9999。化合物1-3的检测限和定量限分别为0.06-0.13 μg/mL和0.21-0.42 μg/mL。化合物1-3的回收率为97.27-103.00%。日内和日间相对标准偏差分别为0.09-0.97%和0.09-1.06%。化合物1-3的含量分别为17.32、28.26和0.01 mg/g。
所建立并经验证的HPLC-PDA方法可能有助于木香这种草药的质量控制。
