Rao Vadaparthi P R, Kumar K, Sarma Vanka Uma Maheswara, Hussain Qazi Altaf, Babu K Suresh
Division of Natural Products Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.
Department of Horticulture, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore, J and K, India.
Pharmacogn Mag. 2015 Jan-Mar;11(41):180-90. doi: 10.4103/0973-1296.149736.
Saussurea lappa is one of the popular Ayurvedic herb; costunolide and dehydrocostus lactones are well-known sesquiterpene lactones contained in many plants used as popular herbs, such as S. lappa, and have been considered as potential candidates for the treatment of various types of tumor.
The present study was used for the quantification of costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations, stability studies of markers and characterization of their degradants.
HPLC analysis was performed on Waters NOVAPAK HR C18 column (300 mm × 3.9 mm i.d., 6 μm) using isocratic elution with acetonitrile and water (60:40% v/v).
The calibration curves of both analytes showed good linearity within the established range 5-100 μg/ml. The limits of detection (LOD) and quantification (LOQ) were 1.5 and 4.6 μg/ml for costunolide and 1.3 and 4.0 μg/ml for dehydrocostus lactone, respectively. Good results were achieved with respect to repeatability (%RSD < 2.0) and recovery (99.3-101.8%).
The method was found to be precise, accurate, specific, and was successfully used for analyzing costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations. The developed method was found to be suitable for stability studies of markers and characterization of their degradation products.
木香是一种常用的阿育吠陀草药；木香烃内酯和去氢木香内酯是许多用作草药的植物（如木香）中含有的著名倍半萜内酯，并且被认为是治疗各种类型肿瘤的潜在候选药物。
本研究用于定量木香及其复方草药制剂中的木香烃内酯和去氢木香内酯，对标志物进行稳定性研究并对其降解产物进行表征。
采用Waters NOVAPAK HR C18柱（300 mm×3.9 mm内径，6μm）进行高效液相色谱分析，使用乙腈和水（60:40% v/v）等度洗脱。
两种分析物的校准曲线在5 - 100μg/ml的设定范围内均显示出良好的线性关系。木香烃内酯的检测限（LOD）和定量限（LOQ）分别为1.5和4.6μg/ml，去氢木香内酯的检测限和定量限分别为1.3和4.0μg/ml。在重复性（%RSD < 2.0）和回收率（99.3 - 101.8%）方面取得了良好的结果。
该方法被发现具有精确、准确、特异的特点，并成功用于分析木香及其复方草药制剂中的木香烃内酯和去氢木香内酯。所建立的方法被发现适用于标志物的稳定性研究及其降解产物的表征。
