Araújo Camila de Souza, de Oliveira Ana Paula, Lima Rafaely Nascimento, Alves Péricles Barreto, Diniz Tâmara Coimbra, da Silva Almeida Jackson Roberto Guedes
Center for Studies and Research of Medicinal Plants, Federal University of Vale do S o Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
Department of Chemistry, Federal University of Sergipe, Campus Prof. Alo sio de Campos, 49.100-000, S o Crist v o, Sergipe, Brazil.
Pharmacogn Mag. 2015 Jul-Sep;11(43):615-8. doi: 10.4103/0973-1296.160462.
Annona vepretorum (AV) is a native tree from Caatinga biome (semiarid region of Brazil) popularly known as "araticum" and "pinha da Caatinga."
This study was carried out to evaluate the chemical constituents and antioxidant activity (AA) of the essential oil from the leaves from AV (EO-Av) collected in Petrolina, Pernambuco, Brazil.
Fresh leaves of AV were cut into pieces, and subjected to distillation for 2 h in a clevenger-type apparatus. Gas chromatograph (GC) analyses were performed using a mass spectrometry/flame ionization detector. The identification of the constituents was assigned on the basis of comparison of their relative retention indices. The antioxidant ability of the EO was investigated through two in vitro models such as radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl method and β-carotene-linoleate-model system. The positive controls (ascorbic acid, butylated hydroxyanisole and butylated hydroxytoluene) were those using the standard solutions. Assays were carried out in triplicate.
The oil showed a total of 21 components, and 17 were identified, representing 93.9% of the crude EO. Spathulenol (43.7%), limonene (20.5%), caryophyllene oxide (8.1%) and α-pinene (5.5%) were found to be the major individual constituents. Spathulenol and caryophyllene oxide could be considered chemotaxonomic markers of these genera. The EO demonstrated weak AA.
番荔枝（Annona vepretorum，AV）是一种原产于卡廷加生物群落（巴西半干旱地区）的树木，俗称“阿拉蒂库姆”和“卡廷加松果”。
本研究旨在评估从巴西伯南布哥州佩特罗利纳采集的番荔枝叶精油（EO-Av）的化学成分和抗氧化活性（AA）。
将番荔枝的新鲜叶子切成小块，在克利夫兰型装置中蒸馏2小时。使用质谱/火焰离子化检测器进行气相色谱（GC）分析。根据相对保留指数的比较确定成分的鉴定。通过两种体外模型研究EO的抗氧化能力，如使用2,2-二苯基-1-苦基肼法的自由基清除活性和β-胡萝卜素-亚油酸酯模型系统。阳性对照（抗坏血酸、丁基羟基茴香醚和丁基羟基甲苯）使用标准溶液。测定重复三次。
该油共有21种成分，鉴定出17种，占粗EO的93.9%。发现spathulenol（43.7%）、柠檬烯（20.5%）、氧化石竹烯（8.1%）和α-蒎烯（5.5%）是主要的单一成分。spathulenol和氧化石竹烯可被视为这些属的化学分类标记。EO表现出较弱的AA。
