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一种用于电化学免疫分析的集成纳米结构微电极的数字微流控装置。

A digital microfluidic device with integrated nanostructured microelectrodes for electrochemical immunoassays.

作者信息

Rackus Darius G, Dryden Michael D M, Lamanna Julian, Zaragoza Alexandre, Lam Brian, Kelley Shana O, Wheeler Aaron R

机构信息

Department of Chemistry, University of Toronto, 80 St. George St., Toronto, ON M5S 3H6, Canada.

出版信息

Lab Chip. 2015;15(18):3776-84. doi: 10.1039/c5lc00660k.

Abstract

Nanostructured microelectrodes (NMEs) are three-dimensional electrodes that have superb sensitivity for electroanalysis. Here we report the integration of NMEs with the versatile fluid-handling system digital microfluidics (DMF), for eventual application to distributed diagnostics outside of the laboratory. In the new methods reported here, indium tin oxide DMF top plates were modified to include Au NMEs as well as counter and pseudoreference electrodes. The new system was observed to outperform planar sensing electrodes of the type that are typically integrated with DMF. A rubella virus (RV) IgG immunoassay was developed to evaluate the diagnostic potential for the new system, relying on magnetic microparticles coated with RV particles and analysis by differential pulse voltammetry. The limit of detection of the assay (0.07 IU mL(-1)) was >100× below the World Health Organization defined cut-off for rubella immunity. The sensitivity of the integrated device and its small size suggest future utility for distributed diagnostics.

摘要

纳米结构微电极(NMEs)是对电分析具有卓越灵敏度的三维电极。在此,我们报告了NMEs与通用流体处理系统数字微流控(DMF)的集成,最终用于实验室外的分布式诊断。在本文报道的新方法中,对氧化铟锡DMF顶板进行了修饰,使其包含金NMEs以及对电极和伪参比电极。观察到新系统优于通常与DMF集成的那种平面传感电极。开发了一种风疹病毒(RV)IgG免疫测定法,以评估新系统的诊断潜力,该方法依靠涂有RV颗粒的磁性微粒并通过差分脉冲伏安法进行分析。该测定法的检测限(0.07 IU mL⁻¹)比世界卫生组织定义的风疹免疫临界值低100多倍。集成设备的灵敏度及其小尺寸表明其在分布式诊断方面具有未来应用价值。

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