Department of Biomaterials and Tissue Engineering, School of Advanced Technology in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Systematic Review and Meta-analysis Expert Group (SRMEG), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
Rev Med Virol. 2021 Jan;31(1):1-11. doi: 10.1002/rmv.2154. Epub 2020 Aug 26.
There is a long way to go before the coronavirus disease 2019 (Covid-19) outbreak comes under control. qRT-PCR is currently used for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Covid-19, but it is expensive, time-consuming, and not as sensitive as it should be. Finding a rapid, easy-to-use, and cheap diagnostic method is necessary to help control the current outbreak. Microfluidic systems provide a platform for many diagnostic tests, including RT-PCR, RT-LAMP, nested-PCR, nucleic acid hybridization, ELISA, fluorescence-Based Assays, rolling circle amplification, aptamers, sample preparation multiplexer (SPM), Porous Silicon Nanowire Forest, silica sol-gel coating/bonding, and CRISPR. They promise faster, cheaper, and easy-to-use methods with higher sensitivity, so microfluidic devices have a high potential to be an alternative method for the detection of viral RNA. These devices have previously been used to detect RNA viruses such as H1N1, Zika, HAV, HIV, and norovirus, with acceptable results. This paper provides an overview of microfluidic systems as diagnostic methods for RNA viruses with a focus on SARS-CoV-2.
在控制 2019 年冠状病毒病（COVID-19）爆发之前，还有很长的路要走。qRT-PCR 目前用于检测 COVID-19 的病原体严重急性呼吸综合征冠状病毒 2（SARS-CoV-2），但它既昂贵又耗时，而且灵敏度不如预期。寻找一种快速、易用且廉价的诊断方法对于帮助控制当前的疫情是必要的。微流控系统为许多诊断测试提供了一个平台，包括 RT-PCR、RT-LAMP、嵌套-PCR、核酸杂交、ELISA、基于荧光的测定、滚环扩增、适体、样品制备多路复用器 (SPM)、多孔硅纳米线森林、硅溶胶-凝胶涂层/键合和 CRISPR。它们承诺具有更高的灵敏度、更快、更便宜和更易于使用的方法，因此微流控设备具有成为病毒 RNA 检测替代方法的巨大潜力。这些设备以前曾用于检测 RNA 病毒，如 H1N1、寨卡病毒、HAV、HIV 和诺如病毒，结果令人满意。本文概述了微流控系统作为 RNA 病毒的诊断方法，重点介绍了 SARS-CoV-2。
