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用于组织工程应用的羟基磷灰石/β-TCP/壳聚糖复合材料的开发和特性研究。

Development and characterization of hydroxyapatite/β-TCP/chitosan composites for tissue engineering applications.

机构信息

Department of Food Sciences, University of Otago, Dunedin, New Zealand.

Department of Food Sciences, University of Otago, Dunedin, New Zealand.

出版信息

Mater Sci Eng C Mater Biol Appl. 2015 Nov 1;56:481-93. doi: 10.1016/j.msec.2015.07.004. Epub 2015 Jul 14.


DOI:10.1016/j.msec.2015.07.004
PMID:26249618
Abstract

Calcium phosphate ceramics that mimic bone composition provide interesting possibilities for the advancement in bone tissue engineering. The present study reports on a chitosan composite reinforced by hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) obtained from waste mussel shells and cross-linked using tripolyphosphate (TPP). The ratios of the ceramic components in composites were 20/10/70, 30/20/50 and 40/30/30 (HA/β-TCP/CH, w/w %). Biodegradation rate, structural properties and in-vitro degradation of the bone-like composite scaffolds were investigated. The optimum amount of TPP required for composite was 2.5% and glycerol was used as plasticizer at an optimized concentration of 1%. Tripolyphosphate cross-linked chitosan composites were developed by freezing and lyophilisation. The Young's modulus of the scaffolds was increased from 4kPa to 17kPa and the porosity of composites dropped from 85 to 68% by increasing the HA/β-TCP ratio. After 28days in physiological solution, bone-like composite scaffolds with a higher ratio of HA/β-TCP (e.g. 40/30/30) showed about 2% lower biodegradation in comparison to scaffolds with a lower ratio of HA/β-TCP (i.e. 20/10/70). The obtained data suggest that the chitosan based bone-like composites could be potential candidates for biomedical applications.

摘要

模拟骨成分的磷酸钙陶瓷为骨组织工程的发展提供了有趣的可能性。本研究报告了一种壳聚糖复合材料,该复合材料由贻贝壳废物中获得的羟基磷灰石(HA)和 β-磷酸三钙(β-TCP)增强,并使用三聚磷酸钠(TPP)交联。复合材料中陶瓷成分的比例为 20/10/70、30/20/50 和 40/30/30(HA/β-TCP/CH,w/w%)。研究了骨样复合支架的降解速率、结构性能和体外降解。复合材料所需的 TPP 最佳用量为 2.5%,甘油在优化浓度 1%下用作增塑剂。通过冷冻和冻干制备了 TPP 交联壳聚糖复合材料。支架的杨氏模量从 4kPa 增加到 17kPa,并且通过增加 HA/β-TCP 的比例,复合材料的孔隙率从 85%降低到 68%。在生理溶液中 28 天后,与 HA/β-TCP 比例较低(例如 20/10/70)的支架相比,HA/β-TCP 比例较高(例如 40/30/30)的骨样复合支架的降解率低约 2%。所得数据表明,基于壳聚糖的骨样复合材料可能是生物医学应用的潜在候选材料。

相似文献

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Development and characterization of hydroxyapatite/β-TCP/chitosan composites for tissue engineering applications.

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[2]
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[3]
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Int J Mol Sci. 2025-4-29

[2]
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Int J Mol Sci. 2024-5-30

[3]
Chitosan (CS)/Hydroxyapatite (HA)/Tricalcium Phosphate (β-TCP)-Based Composites as a Potential Material for Pulp Tissue Regeneration.

Polymers (Basel). 2023-7-28

[4]
Effect of Selected Crosslinking and Stabilization Methods on the Properties of Porous Chitosan Composites Dedicated for Medical Applications.

Polymers (Basel). 2023-5-29

[5]
Application Progress of Modified Chitosan and Its Composite Biomaterials for Bone Tissue Engineering.

Int J Mol Sci. 2022-6-12

[6]
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Prog Biomater. 2022-9

[7]
Bone Bricks: The Effect of Architecture and Material Composition on the Mechanical and Biological Performance of Bone Scaffolds.

ACS Omega. 2022-2-22

[8]
Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration.

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[9]
Improvement of physico-chemical properties of dextran-chitosan composite scaffolds by addition of nano-hydroxyapatite.

Sci Rep. 2018-8-15

[10]
A facile, efficient, and sustainable chitosan/CaHAp catalyst and one-pot synthesis of novel 2,6-diamino-pyran-3,5-dicarbonitriles.

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