Biernat Monika, Woźniak Anna, Chraniuk Milena, Panasiuk Mirosława, Tymowicz-Grzyb Paulina, Pagacz Joanna, Antosik Agnieszka, Ciołek Lidia, Gromadzka Beata, Jaegermann Zbigniew
Biomaterials Research Group, Łukasiewicz Research Network-Institute of Ceramics and Building Materials, Cementowa 8, 31-983 Kraków, Poland.
Department of In Vitro Studies, Institute of Biotechnology and Molecular Medicine, Kampinoska 25, 80-180 Gdańsk, Poland.
Polymers (Basel). 2023 May 29;15(11):2507. doi: 10.3390/polym15112507.
Chitosan is one of the most commonly employed natural polymers for biomedical applications. However, in order to obtain stable chitosan biomaterials with appropriate strength properties, it is necessary to subject it to crosslinking or stabilization. Composites based on chitosan and bioglass were prepared using the lyophilization method. In the experimental design, six different methods were used to obtain stable, porous chitosan/bioglass biocomposite materials. This study compared the crosslinking/stabilization of chitosan/bioglass composites with ethanol, thermal dehydration, sodium tripolyphosphate, vanillin, genipin, and sodium β-glycerophosphate. The physicochemical, mechanical, and biological properties of the obtained materials were compared. The results showed that all the selected crosslinking methods allow the production of stable, non-cytotoxic porous composites of chitosan/bioglass. The composite with genipin stood out with the best of the compared properties, taking into account biological and mechanical characteristics. The composite stabilized with ethanol is distinct in terms of its thermal properties and swelling stability, and it also promotes cell proliferation. Regarding the specific surface area, the highest value exposes the composite stabilized by the thermal dehydration method.
壳聚糖是生物医学应用中最常用的天然聚合物之一。然而，为了获得具有适当强度特性的稳定壳聚糖生物材料，有必要对其进行交联或稳定化处理。采用冻干法制备了基于壳聚糖和生物玻璃的复合材料。在实验设计中，使用了六种不同的方法来获得稳定的多孔壳聚糖/生物玻璃生物复合材料。本研究比较了壳聚糖/生物玻璃复合材料与乙醇、热脱水、三聚磷酸钠、香草醛、京尼平及β-甘油磷酸钠的交联/稳定化情况。对所得材料的物理化学、力学和生物学性能进行了比较。结果表明，所有选定的交联方法都能制备出稳定的、无细胞毒性的多孔壳聚糖/生物玻璃复合材料。考虑到生物学和力学特性，京尼平交联的复合材料在比较的性能中表现最佳。乙醇稳定化的复合材料在热性能和溶胀稳定性方面具有独特性，并且还能促进细胞增殖。就比表面积而言，热脱水法稳定化的复合材料具有最高值。
