Bhat H K, Ansari G A
Division of Biochemistry, University of Texas Medical Branch, Galveston 77550.
J Chromatogr. 1989 Dec 8;483:369-78. doi: 10.1016/s0021-9673(01)93136-x.
Methods for the separation of ethyl- and 2-chloroethyl esters of fatty acids using argentation thin-layer chromatography (TLC) and linear and circular reversed-phase TLC are described. An argentation TLC method for the separation of cholesterol esters is also described. The separation is based on the degree of unsaturation in the fatty acid moiety. In all instances good and reproducible resolution was achieved by unidirectional single developments at room temperature with reduced analysis times (9 min for argentation TLC, 7 min for circular reversed-phase TLC).
描述了使用银化薄层色谱法（TLC）以及线性和圆形反相TLC分离脂肪酸乙酯和2-氯乙酯的方法。还描述了一种用于分离胆固醇酯的银化TLC方法。分离基于脂肪酸部分的不饱和度。在所有情况下，通过在室温下单向单次展开，在缩短的分析时间内（银化TLC为9分钟，圆形反相TLC为7分钟）实现了良好且可重现的分离度。
