化学凝集素学：用于在体内探测哺乳动物凝集素的配体和动力学的工具。
Chemical Lectinology: Tools for Probing the Ligands and Dynamics of Mammalian Lectins In Vivo.
作者信息
Belardi Brian, Bertozzi Carolyn R
机构信息
Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
Department of Chemistry and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305-4401, USA.
出版信息
Chem Biol. 2015 Aug 20;22(8):983-93. doi: 10.1016/j.chembiol.2015.07.009. Epub 2015 Aug 6.
The importance and complexity associated with the totality of glycan structures, i.e. the glycome, has garnered significant attention from chemists and biologists alike. However, what is lacking from this biochemical picture is how cells, tissues, and organisms interpret glycan patterns and translate this information into appropriate responses. Lectins, glycan-binding proteins, are thought to bridge this gap by decoding the glycome and dictating cell fate based on the underlying chemical identities and properties of the glycome. Yet, our understanding of the in vivo ligands and function for most lectins is still incomplete. This review focuses on recent advances in chemical tools to study the specificity and dynamics of mammalian lectins in live cells. A picture emerges of lectin function that is highly sensitive to its organization, which in turn drastically shapes immunity and cancer progression. We hope this review will inspire biologists to make use of these new techniques and stimulate chemists to continue developing innovative approaches to probe lectin biology in vivo.
聚糖结构的整体，即糖组，所具有的重要性和复杂性已经引起了化学家和生物学家的极大关注。然而，在这种生物化学图景中缺少的是细胞、组织和生物体如何解读聚糖模式并将这些信息转化为适当反应。凝集素，即聚糖结合蛋白，被认为通过解码糖组并基于糖组潜在的化学特性和性质来决定细胞命运，从而弥补这一差距。然而，我们对大多数凝集素在体内的配体和功能的理解仍然不完整。本综述聚焦于用于研究活细胞中哺乳动物凝集素特异性和动力学的化学工具的最新进展。一幅关于凝集素功能的图景浮现出来，它对其组织高度敏感，而这又反过来极大地塑造了免疫和癌症进展。我们希望本综述能激励生物学家利用这些新技术，并刺激化学家继续开发创新方法以在体内探究凝集素生物学。
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