牙齿DNA分析及其在法医牙科学中的应用。
Analysis of DNA from tooth and application to forensic dental medicine.
作者信息
Yamada Y, Yamamoto K, Yoshii T, Ishiyama I
出版信息
Nihon Hoigaku Zasshi. 1989 Oct;43(5):420-3.
PMID:2625734
Abstract
The DNA extracted from teeth was examined to find out the usefulness in forensic dental medicine. Samples of tooth-derived DNA were digested with a restriction enzyme, electrophosed through agarose gel and subjected to Southern blot hybridization, using a 32P-labeled minisatellite DNA probe "Myo" and a Y chromosome-specific DNA probe. The results indicate that DNA extracted from dental pulp is useful for identification of individuals, paternity testing, and sex determination.
摘要
对从牙齿中提取的DNA进行检测，以确定其在法医牙科学中的实用性。将牙齿来源的DNA样本用限制性内切酶消化，通过琼脂糖凝胶电泳，然后使用32P标记的小卫星DNA探针“Myo”和Y染色体特异性DNA探针进行Southern印迹杂交。结果表明，从牙髓中提取的DNA可用于个体识别、亲子鉴定和性别鉴定。
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