Landarani-Isfahani Amir, Taheri-Kafrani Asghar, Amini Mina, Mirkhani Valiollah, Moghadam Majid, Soozanipour Asieh, Razmjou Amir
Catalysis Division, Department of Chemistry, and ‡Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan , Isfahan 81746-73441, Iran.
Langmuir. 2015 Aug 25;31(33):9219-27. doi: 10.1021/acs.langmuir.5b02004. Epub 2015 Aug 14.
Although several strategies are now available for immobilization of enzymes to magnetic nanoparticles for bioapplications, little progresses have been reported on the use of dendritic or hyperbranched polymers for the same purpose. Herein, we demonstrated synthesis of magnetic nanoparticles supported hyperbranched polyglycerol (MNP/HPG) and a derivative conjugated with citric acid (MNP/HPG-CA) as unique and convenient nanoplatforms for immobilization of enzymes. Then, an important industrial enzyme, xylanase, was immobilized on the nanocarriers to produce robust biocatalysts. A variety of analytical tools were used to study the morphological, structural, and chemical properties of the biocatalysts. Additionally, the results of biocatalyst systems exhibited the substantial improvement of reactivity, reusability, and stability of xylanase due to this strategy, which might confer them a wider range of applications.
尽管目前有几种策略可用于将酶固定在磁性纳米颗粒上以用于生物应用，但关于使用树枝状或超支化聚合物实现相同目的的报道却很少。在此，我们展示了磁性纳米颗粒负载的超支化聚甘油（MNP/HPG）以及与柠檬酸共轭的衍生物（MNP/HPG-CA）的合成，它们是用于固定酶的独特且便捷的纳米平台。然后，将一种重要的工业酶木聚糖酶固定在纳米载体上，以制备出坚固的生物催化剂。使用了各种分析工具来研究生物催化剂的形态、结构和化学性质。此外，生物催化剂系统的结果表明，由于该策略，木聚糖酶的反应性、可重复使用性和稳定性有了显著提高，这可能使其具有更广泛的应用范围。
