Jia Yuying, Yao Xingdong, Zhao Mingzhe, Zhao Qiang, Du Yanli, Yu Cuimei, Xie Futi
Soybean Research Institute, Shenyang Agricultural University, Shenyang 110866, China.
Int J Mol Sci. 2015 Aug 7;16(8):18522-43. doi: 10.3390/ijms160818522.
The susceptibility of soybean genotype to Agrobacterium infection is a key factor for the high level of genetic transformation efficiency. The objective of this study is to evaluate the plant factors related to transformation in cotyledonary nodes during the Agrobacterium infection process. This study selected three genotypes (Williams 82, Shennong 9 and Bert) with high transformation efficiency, which presented better susceptibility to Agrobacterium infection, and three low transformation efficiency genotypes (General, Liaodou 16 and Kottman), which showed a relatively weak susceptibility. Gibberellin (GA) levels and soybean GA20ox2 and CYP707A2 transcripts of high-efficiency genotypes increased and were higher than those of low-efficiency genotypes; however, the opposite performance was shown in abscisic acid (ABA). Higher zeatin riboside (ZR) content and DNA quantity, and relatively higher expression of soybean IPT5, CYCD3 and CYCA3 were obtained in high-efficiency genotypes. High-efficiency genotypes had low methyl jasmonate (MeJA) content, polyphenol oxidase (PPO) and peroxidase (POD) activity, and relatively lower expression of soybean OPR3, PPO1 and PRX71. GA and ZR were positive plant factors for Agrobacterium-mediated soybean transformation by facilitating germination and growth, and increasing the number of cells in DNA synthesis cycle, respectively; MeJA, PPO, POD and ABA were negative plant factors by inducing defence reactions and repressing germination and growth, respectively.
大豆基因型对农杆菌感染的敏感性是遗传转化效率高水平的关键因素。本研究的目的是评估农杆菌感染过程中与子叶节转化相关的植物因素。本研究选择了三种转化效率高、对农杆菌感染表现出更好敏感性的基因型（Williams 82、神农9和Bert），以及三种转化效率低、敏感性相对较弱的基因型（General、辽豆16和Kottman）。高效基因型的赤霉素（GA）水平以及大豆GA20ox2和CYP707A2转录本增加，且高于低效基因型；然而，脱落酸（ABA）则表现出相反的情况。高效基因型中获得了更高的玉米素核苷（ZR）含量和DNA量，以及大豆IPT5、CYCD3和CYCA3相对较高的表达。高效基因型的茉莉酸甲酯（MeJA）含量、多酚氧化酶（PPO）和过氧化物酶（POD）活性较低，以及大豆OPR3、PPO1和PRX71相对较低的表达。GA和ZR分别通过促进萌发和生长以及增加DNA合成周期中的细胞数量，成为农杆菌介导的大豆转化的正向植物因素；MeJA、PPO、POD和ABA分别通过诱导防御反应和抑制萌发及生长，成为负向植物因素。
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