Meurer C A, Dinkins R D, Collins G B
Department of Agronomy, N109 Agriculture Science Center Building North, University of Kentucky, Lexington, KY 40546-0091, USA Fax: +1-606-257-7125 e-mail:
Plant Cell Rep. 1998 Dec;18(3-4):180-186. doi: 10.1007/s002990050553.
Cotyledonary node transformation efficiency was evaluated using a sonication assisted Agrobacterium-mediated transformation (SAAT) protocol, three dissimilar A. tumefaciens strains, and explants derived from 28 diverse cultivars and/or genotypes of soybean [Glycine max (L.) Merr.]. The explants were evaluated at 10 and 45 days after co-cultivation for transformation with a binary vector containing both a GUS-intron gene and an NPTII selectable marker. The best overall strain of A. tumefaciens was determined to be KYRT1 based on stable GUS transformation of soybean cotyledonary node explants measured at the terminal 45 day evaluation point. SAAT did not increase stable transformation at 45 days post co-cultivation. SAAT was determined to significantly decrease shoot proliferation of some genotypes, but it is unclear what effect this may have on the recovery of transformed shoots. Significant differences were also detected between genotypes for transformation and shoot proliferation frequency.
利用超声辅助农杆菌介导转化（SAAT）方案、三种不同的根癌农杆菌菌株以及源自28个不同大豆[Glycine max (L.) Merr.]品种和/或基因型的外植体，评估子叶节转化效率。共培养10天和45天后，对外植体进行评估，以检测其是否被含有GUS内含子基因和NPTII选择标记的二元载体转化。根据在45天终末评估点测得的大豆子叶节外植体稳定GUS转化情况，确定根癌农杆菌的最佳总体菌株为KYRT1。SAAT在共培养45天后并未提高稳定转化率。已确定SAAT会显著降低某些基因型的芽增殖，但尚不清楚这对转化芽的恢复可能有何影响。在转化和芽增殖频率方面，不同基因型之间也检测到显著差异。
