Patil Rajeshwari H, Babu R L, Naveen Kumar M, Kiran Kumar K M, Hegde Shubha M, Nagesh Rashmi, Ramesh Govindarajan T, Sharma S Chidananda
Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru, 560 056, Karnataka, India.
Department of Bioinformatics and Biotechnology, Karnataka State Women's University, Jnanashakthi Campus, Vijayapura, 586 108, Karnataka, India.
Inflammation. 2016 Feb;39(1):138-147. doi: 10.1007/s10753-015-0232-z.
Apigenin is one of the plant flavonoids present in fruits and vegetables, acting as an important nutraceutical component. It is recognized as a potential antioxidant, antimicrobial, and anti-inflammatory molecule. In the present study, the mechanism of anti-inflammatory action of apigenin on lipopolysaccharide (LPS)-induced pro-inflammatory cytokines and activator protein-1 (AP-1) factors in human lung A549 cells was investigated. The anti-inflammatory activity of apigenin on LPS-induced inflammation was determined by analyzing the expression of pro-inflammatory cytokines, nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and different AP-1 factors. Apigenin significantly inhibited the LPS-induced expression of iNOS, COX-2, expression of pro-inflammatory cytokines (IL-1β, IL-2, IL-6, IL-8, and TNF-α), and AP-1 proteins (c-Jun, c-Fos, and JunB) including nitric oxide production. Study confirms the anti-inflammatory effect of apigenin by inhibiting the expression of inflammatory mediators and AP-1 factors involved in the inflammation and its importance in the treatment of lung inflammatory diseases.
芹菜素是水果和蔬菜中存在的植物类黄酮之一，是一种重要的营养成分。它被认为是一种潜在的抗氧化、抗菌和抗炎分子。在本研究中，研究了芹菜素对人肺A549细胞中脂多糖（LPS）诱导的促炎细胞因子和活化蛋白-1（AP-1）因子的抗炎作用机制。通过分析促炎细胞因子、一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）和不同AP-1因子的表达，确定了芹菜素对LPS诱导炎症的抗炎活性。芹菜素显著抑制LPS诱导的iNOS、COX-2表达、促炎细胞因子（IL-1β、IL-2、IL-6、IL-8和TNF-α）表达以及AP-1蛋白（c-Jun、c-Fos和JunB）表达，包括一氧化氮的产生。研究证实了芹菜素通过抑制参与炎症的炎症介质和AP-1因子的表达而具有抗炎作用，以及其在治疗肺部炎症性疾病中的重要性。
