在资源有限的高负担人类免疫缺陷病毒和结核病环境中，将Xpert MTB/RIF纳入通过免疫表型分析排除淋巴瘤而提交的液体标本诊断算法中的获益情况。
A High Burden Human Immunodeficiency Virus and Tuberculosis Resource Limited Setting, Gains from Including Xpert MTB/RIF in the Diagnostic Algorithm of Fluid Specimens Submitted for Exclusion of Lymphoma by Immunophenotypic Analysis.
作者信息
Kilfoil Kim Michelle, Mayne Elizabeth, Scott Lesley, Stevens Wendy
机构信息
Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Science, University of the Witwatersrand, Johannesburg, South Africa.
Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Science, University of the Witwatersrand, Johannesburg, South Africa; National Health Laboratory Service, National Priority Program, Johannesburg, South Africa.
出版信息
PLoS One. 2015 Aug 17;10(8):e0134404. doi: 10.1371/journal.pone.0134404. eCollection 2015.
Abstract
This study investigated the benefit of incorporating Xpert MTB/RIF into the diagnostic algorithm of fluid specimens received for immunophenotypic analysis to exclude lymphoma. It was found that in a high burden HIV/TB setting, like South Africa, 130/229 (57%) of fluid specimens referred for immunophenotypic analysis to exclude lymphoma are not referred for concurrent MTBC liquid culture testing by the treating clinician. Of 99/229 (43%) specimens with corresponding culture results, Xpert sensitivity and specificity were 50% (CI:26-75%) and 99% (CI:91-100%) respectively. This demonstrates that incorporation of Xpert into the laboratory diagnostic algorithm in the immunophenotypic laboratory would improve patient work-up and care.
摘要
本研究调查了将Xpert MTB/RIF纳入用于免疫表型分析以排除淋巴瘤的体液标本诊断算法中的益处。研究发现，在南非这样的高负担HIV/TB环境中，为免疫表型分析以排除淋巴瘤而送检的229份体液标本中，有130份（57%）未被治疗医生同时送检结核分枝杆菌液体培养检测。在229份中有相应培养结果的99份（43%）标本中，Xpert的敏感性和特异性分别为50%（CI：26-75%）和99%（CI：91-100%）。这表明在免疫表型实验室将Xpert纳入实验室诊断算法将改善患者的检查和护理。
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