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一种新型红苹果品种对乳腺癌细胞增殖和体外肿瘤发生的抑制作用

Inhibition of Breast Cancer Cell Proliferation and In Vitro Tumorigenesis by a New Red Apple Cultivar.

作者信息

Schiavano Giuditta Fiorella, De Santi Mauro, Brandi Giorgio, Fanelli Mirco, Bucchini Anahi, Giamperi Laura, Giomaro Giovanna

机构信息

Department of Biomolecular Sciences, University of Urbino "Carlo Bo", Urbino (PU), Italy.

Department of Earth, Life and Environmental Sciences, University of Urbino "Carlo Bo", Urbino (PU), Italy.

出版信息

PLoS One. 2015 Aug 18;10(8):e0135840. doi: 10.1371/journal.pone.0135840. eCollection 2015.

DOI:10.1371/journal.pone.0135840
PMID:26284516
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4540469/
Abstract

PURPOSE

The aim of this study was to evaluate the antiproliferative activity in breast cancer cells and the inhibition of tumorigenesis in pre-neoplastic cells of a new apple cultivar with reddish pulp, called the Pelingo apple.

METHODS

The antiproliferative activity was evaluated in MCF-7 and MDA-MB-231 human breast cancer cells. The inhibition of tumorigenesis was performed in JB6 promotion-sensitive (P+) cells.

RESULTS

Results showed that Pelingo apple juice is characterized by a very high polyphenol content and strongly inhibited breast cancer cell proliferation. Its antiproliferative activity was found to be higher than the other five apple juices tested. Pelingo juice induced cell accumulation in the G2/M phase of the cell cycle and autophagy through overexpression of p21, inhibition of extracellular signal-regulated kinases 1/2 (ERK1/2) activity and an increase in lipidated microtubule-associated protein-1 light chain-3 beta (LC3B). Remarkably, Pelingo juice inhibited the 12-o-tetra-decanoyl-phorbol-13-acetate (TPA)-induced tumorigenesis of JB6 P+ cells, suppressing colony formation in semi-solid medium and TPA-induced ERK1/2 phosphorylation.

CONCLUSIONS

Our data indicate that the Pelingo apple is rich in food components that can markedly inhibit in vitro tumorigenesis and growth of human breast cancer cells and could provide natural bioactive non-nutrient compounds, with potential chemopreventive activity.

摘要

目的

本研究旨在评估一种名为佩林戈苹果的新型红果肉苹果品种对乳腺癌细胞的抗增殖活性以及对癌前细胞肿瘤发生的抑制作用。

方法

在MCF - 7和MDA - MB - 231人乳腺癌细胞中评估抗增殖活性。在对促进敏感的JB6（P +）细胞中进行肿瘤发生抑制实验。

结果

结果表明，佩林戈苹果汁的特点是多酚含量非常高，能强烈抑制乳腺癌细胞增殖。其抗增殖活性高于所测试的其他五种苹果汁。佩林戈果汁通过p21的过表达、细胞外信号调节激酶1/2（ERK1/2）活性的抑制以及脂化微管相关蛋白1轻链3β（LC3B）的增加，诱导细胞周期在G2/M期积累和自噬。值得注意的是，佩林戈果汁抑制了12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导的JB6 P +细胞肿瘤发生，抑制了半固体培养基中的集落形成和TPA诱导的ERK1/2磷酸化。

结论

我们的数据表明，佩林戈苹果富含能够显著抑制人乳腺癌细胞体外肿瘤发生和生长的食物成分，并能提供具有潜在化学预防活性的天然生物活性非营养化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/07d7a9a21db9/pone.0135840.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/4454ab81641a/pone.0135840.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/38ba4f720803/pone.0135840.g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/0b24a1aa491c/pone.0135840.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/054a7baa609d/pone.0135840.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/07d7a9a21db9/pone.0135840.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/4454ab81641a/pone.0135840.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/38ba4f720803/pone.0135840.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/fb3795950c8d/pone.0135840.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/0b24a1aa491c/pone.0135840.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/054a7baa609d/pone.0135840.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c9/4540469/07d7a9a21db9/pone.0135840.g006.jpg

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