白猪屎豆中的异黄酮类化合物通过抑制PPAR-γ信号通路抑制3T3-L1细胞的脂肪细胞分化和脂质积累。
Isoflavonoids from Crotalaria albida Inhibit Adipocyte Differentiation and Lipid Accumulation in 3T3-L1 Cells via Suppression of PPAR-γ Pathway.
作者信息
Sun Qinhu, Chou Guixin
机构信息
The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica of Shanghai University of Traditional Chinese Medicine, Shanghai, China; Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai, China.
出版信息
PLoS One. 2015 Aug 18;10(8):e0135893. doi: 10.1371/journal.pone.0135893. eCollection 2015.
Two 2″-isopropenyl dihydrofuran isoflavonoids (1 and 3), one 2″-isopropenyl dihydrofuran chromone (2), as well as 13 known compounds were isolated from the herbs of Crotalaria albida. Their structures and relative configurations were elucidated via NMR and HRESIMS analyses. The 2″ S absolute configuration of 1 and 2 were deduced by comparing their NOESY spectra with that of 3, which was determined via single crystal X-ray diffraction (CuKα). The 3R absolute configuration of 1 was determined by CD. Compounds 1, 2, and 3 inhibit the adipocyte differentiation and lipid accumulation of 3T3-L1 through down-regulation of PPAR-γ activity.
从白猪屎豆的全草中分离得到两个2″-异丙烯基二氢呋喃异黄酮类化合物（1和3）、一个2″-异丙烯基二氢呋喃色酮（2）以及13个已知化合物。通过核磁共振（NMR）和高分辨电喷雾电离质谱（HRESIMS）分析阐明了它们的结构和相对构型。通过将化合物1和2的核欧沃豪斯效应光谱（NOESY）与通过单晶X射线衍射（CuKα）确定的化合物3的光谱进行比较，推断出化合物1和2的2″S绝对构型。通过圆二色光谱（CD）确定了化合物1的3R绝对构型。化合物1、2和3通过下调过氧化物酶体增殖物激活受体γ（PPAR-γ）的活性来抑制3T3-L1脂肪细胞的分化和脂质积累。
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