Hoang Van-An, Kim Yeon-Ju, Nguyen Ngoc Lan, Kang Chang Ho, Kang Jong-Pyo, Singh Priyanka, Farh Mohamed El-Agamy, Yang Dong-Uk, Yang Deok-Chun
Department of Oriental Medicinal Biotechnology, Kyung Hee University Giheung-gu Yongin-si, Gyeonggi-do, Republic of Korea.
Division of Applied Life Science and PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 660-701, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Sep;65(9):3196-3202. doi: 10.1099/ijsem.0.000399. Epub 2015 Jun 25.
A novel Gram-staining-positive, rod-shaped bacterium, designated DCY100(T), was isolated from rhizome of mountain ginseng root in Hwacheon mountain, Gangwon province, Republic of Korea. The 16S rRNA gene sequence analysis showed that strain DCY100(T) belonged to the genus Microbacterium and was most closely related to Microbacterium ginsengisoli KCTC 19189(T) (97.9%), Microbacterium lacus JCM 15575(T) (97.2%) and Microbacterium invictum DSM 19600(T) (97.1%). The major menaquinones were MK-11 and MK-12. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid. The major fatty acids (>10.0%) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The cell-wall peptidoglycan contained the amino acids ornithine, alanine, glutamic acid and glycine; whole-cell sugars consisted of glucose, galactose, rhamnose and ribose. The DNA G+C content was 63.6 ± 0.7 mol%. The DNA-DNA hybridization relatedness values between strain DCY100(T) and Microbacterium ginsengisoli KCTC 19189(T), Microbacterium lacus JCM 15575(T) and Microbacterium invictum DSM 19600(T) were 36.2 ± 0.4, 22.0 ± 3.0 and 15.3 ± 1.8%, respectively. On the basis of phenotypic, chemotaxonomic and genotypic analyses, the isolate is classified as a representative of a novel species in the genus Microbacterium, for which the name Microbacterium rhizomatis DCY100(T) is proposed. The type strain is DCY100(T) ( = KCTC 39529(T) = JCM 30598(T)).
从韩国江原道华川山的山参根茎中分离出一株新型革兰氏染色阳性、杆状细菌，命名为DCY100(T)。16S rRNA基因序列分析表明，菌株DCY100(T)属于微杆菌属，与参地微杆菌KCTC 19189(T)（97.9%）、湖微杆菌JCM 15575(T)（97.2%）和顽强微杆菌DSM 19600(T)（97.1%）关系最为密切。主要甲基萘醌为MK-11和MK-12。主要极性脂类为双磷脂酰甘油、磷脂酰甘油和一种未鉴定的糖脂。主要脂肪酸（>10.0%）为anteiso-C15 : 0、anteiso-C17 : 0和iso-C16 : 0。细胞壁肽聚糖含有鸟氨酸、丙氨酸、谷氨酸和甘氨酸；全细胞糖由葡萄糖、半乳糖、鼠李糖和核糖组成。DNA G+C含量为63.6±0.7 mol%。菌株DCY100(T)与参地微杆菌KCTC 19189(T)、湖微杆菌JCM 15575(T)和顽强微杆菌DSM 19600(T)之间的DNA-DNA杂交相关性值分别为36.2±0.4%、22.0±3.0%和15.3±1.8%。基于表型、化学分类和基因型分析，该分离株被归类为微杆菌属一个新物种的代表，为此提出了根茎微杆菌DCY100(T)这一名称。模式菌株为DCY100(T)（=KCTC 39529(T)=JCM 30598(T)）。
