Kozuka Miyuki, Yamane Takuya, Nakano Yoshihisa, Nakagaki Takenori, Ohkubo Iwao, Ariga Hiroyoshi
Department of Health and Nutrition, Faculty of Human Science, Hokkaido Bunkyo University, Eniwa 061-1449, Japan.
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan.
Biochem Biophys Res Commun. 2015 Sep 25;465(3):433-6. doi: 10.1016/j.bbrc.2015.08.031. Epub 2015 Aug 18.
Aronia berries have many potential effects on health, including an antioxidant effect, effect for antimutagenesis, hepatoprotection and cardioprotection, an antidiabetic effect and inhibition of cancer cell proliferation. Previous human studies have shown that aronia juice may be useful for treatment of obesity disorders. In this study, we found that aronia juice has an inhibitory effect against dipeptidyl peptidase IV (DPP IV) (EC 3.4.14.5). DPP IV is a peptidase that cleaves the N-terminal region of incretins such as glucagon-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). Inactivation of incretins by DPP IV induces reduction of insulin secretion. Furthermore, we identified that cyanidin 3, 5-diglucoside as the DPP IV inhibitor in aronia juice. DPP IV was inhibited more strongly by cyanidin 3, 5-diglucoside than by cyanidin and cyanidin 3-glucoside. The results suggest that DPP IV is inhibited by cyanidin 3, 5-diglucoside present in aronia juice. The antidiabetic effect of aronia juice may be mediated through DPP IV inhibition by cyanidin 3, 5-diglucoside.
黑果腺肋花楸果实对健康有许多潜在影响，包括抗氧化作用、抗诱变作用、肝脏保护和心脏保护作用、抗糖尿病作用以及抑制癌细胞增殖。先前的人体研究表明，黑果腺肋花楸果汁可能对治疗肥胖症有用。在本研究中，我们发现黑果腺肋花楸果汁对二肽基肽酶IV（DPP IV）（EC 3.4.14.5）具有抑制作用。DPP IV是一种肽酶，可切割肠促胰岛素（如胰高血糖素依赖性促胰岛素多肽（GIP）和胰高血糖素样肽-1（GLP-1））的N端区域。DPP IV使肠促胰岛素失活会导致胰岛素分泌减少。此外，我们确定矢车菊素3,5-二葡萄糖苷是黑果腺肋花楸果汁中的DPP IV抑制剂。矢车菊素3,5-二葡萄糖苷对DPP IV的抑制作用比对矢车菊素和矢车菊素3-葡萄糖苷更强。结果表明，黑果腺肋花楸果汁中存在的矢车菊素3,5-二葡萄糖苷可抑制DPP IV。黑果腺肋花楸果汁的抗糖尿病作用可能是通过矢车菊素3,5-二葡萄糖苷抑制DPP IV来介导的。
