Sundelin Thomas, Strømeng Gunn Mari, Gjærum Halvor B, Amby Daniel Buchvaldt, Ørstad Kari, Jensen Birgit, Lund Ole Søgaard, Stensvand Arne
Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark.
Bioforsk, Norwegian Institute for Agricultural and Environmental Research, Plant Health and Plant Protection Division, 1430 Ås, Norway.
FEMS Microbiol Lett. 2015 Aug;362(16). doi: 10.1093/femsle/fnv130. Epub 2015 Aug 21.
Herbaria collections containing plants with disease symptoms are highly valuable, and they are often the only way to investigate outbreaks and epidemics from the past as the number of viable isolates in culture collections is often limited. Species belonging to the Colletotrichum acutatum complex infect a range of important crops. As members of the C. acutatum complex are easily confused with other Colletotrichum species, molecular methods are central for the correct identification. We performed molecular analyses on 21 herbaria specimens, displaying anthracnose symptoms, collected in Norway and Denmark before the first confirmed findings of C. acutatum complex members in this region. Sequencing parts of the fungal ITS regions showed that members of the species complex were present in 13 of the 21 specimens collected in different parts of Norway and Denmark between 1948 and 1991, representing seven plant hosts (three cherry species, apple, raspberry and rhododendron). This is the first time herbarium specimens have been used to study these pathogens under Nordic conditions. Differences in the ITS sequences suggest the presence of different genotypes within the complex, indicating a well-established population.
含有具有病害症状植物的植物标本馆馆藏非常有价值，而且它们常常是调查过去疫情和流行病爆发情况的唯一途径，因为培养物保藏中心中可存活分离物的数量往往有限。尖孢炭疽菌复合体中的物种会感染一系列重要作物。由于尖孢炭疽菌复合体的成员很容易与其他炭疽菌属物种混淆，分子方法对于正确鉴定至关重要。我们对21份表现出炭疽病症状的植物标本馆标本进行了分子分析，这些标本是在挪威和丹麦在该地区首次确认发现尖孢炭疽菌复合体成员之前采集的。对真菌ITS区域的部分进行测序表明，在1948年至1991年间于挪威和丹麦不同地区采集的21份标本中，有13份含有该物种复合体的成员，代表了七种植物寄主（三种樱桃属物种、苹果、树莓和杜鹃花）。这是首次在北欧条件下利用植物标本馆标本研究这些病原体。ITS序列的差异表明该复合体中存在不同的基因型，这意味着存在一个成熟的种群。
