Hadapad Ashok B, Prabhakar Chandra S, Chandekar Snehal C, Tripathi Jyoti, Hire Ramesh S
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
Department of Entomology, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India.
Pest Manag Sci. 2016 Jun;72(6):1222-30. doi: 10.1002/ps.4102. Epub 2015 Sep 25.
The microbiota plays an important role in insect development and fitness. Understanding the gut microbiota composition is essential for the development of pest management strategies. Midgut bacteria were isolated from nine wild B. cucurbitae populations collected from different agroecological zones of India. These isolates were further studied for attractant potential of fruit fly adults, and the chemical constituents in the supernatants of gut bacteria were analysed.
Twenty-six bacterial isolates belonging to the families Enterobacteriaceae, Bacillaceae, Micrococcaceae and Staphylococcaceae were isolated and identified on the basis of 16S rRNA gene sequence analysis. The dominant species in the midgut of melon fly were from the genera Enterobacter (34.6%), Klebsiella (19.2%), Citrobacter (7.7%), Bacillus (15.4%) and Providencia (7.7%), and 3.8% each of Micrococcus, Staphylococcus, Leclercia and Exiguobacterium. Bactrocera cucurbitae and B. dorsalis adults were significantly attracted to bacterial whole cell cultures and their supernatants in the fruit fly attraction bioassays. Bacillus cereus, Enterobacter, Klebsiella, Citrobacter and Providencia species attracted both male and females of Bactrocera species. The supernatants of Klebsiella, Citrobacter and Providencia species attracted a significantly greater number of females than males. The most abundant chemical constituents in supernatants of K. oxytoca and C. freundii were 3-methyl-1-butanol, 2-phenylethanol, butyl isocyanatoacetate, 2-methyl-1-propanol and 3-hydroxy-2-butanone, as identified by gas chromatography-mass spectrometry.
The bacterial endosymbionts associated with melon fly exhibited attractant potential which could facilitate eco-friendly insect control strategies. © 2015 Society of Chemical Industry.
微生物群在昆虫发育和健康中发挥着重要作用。了解肠道微生物群组成对于制定害虫管理策略至关重要。从印度不同农业生态区采集的9个野生南瓜实蝇种群中分离出中肠细菌。对这些分离菌株进一步研究其对果蝇成虫的引诱潜力，并分析肠道细菌上清液中的化学成分。
基于16S rRNA基因序列分析，分离并鉴定出26株属于肠杆菌科、芽孢杆菌科、微球菌科和葡萄球菌科的细菌分离株。瓜实蝇中肠的优势菌种来自肠杆菌属（34.6%）、克雷伯菌属（19.2%）、柠檬酸杆菌属（7.7%）、芽孢杆菌属（15.4%）和普罗威登斯菌属（7.7%），微球菌属、葡萄球菌属、勒克勒菌属和嗜麦芽寡养单胞菌属各占3.8%。在果蝇引诱生物测定中，南瓜实蝇和橘小实蝇成虫对细菌全细胞培养物及其上清液有显著吸引力。蜡样芽孢杆菌、肠杆菌、克雷伯菌、柠檬酸杆菌和普罗威登斯菌属吸引了橘小实蝇属的雄性和雌性成虫。克雷伯菌属、柠檬酸杆菌属和普罗威登斯菌属的上清液吸引的雌性果蝇数量明显多于雄性。通过气相色谱-质谱法鉴定，产酸克雷伯菌和弗氏柠檬酸杆菌上清液中最丰富的化学成分是3-甲基-1-丁醇、2-苯乙醇、异氰基乙酸丁酯、2-甲基-1-丙醇和3-羟基-2-丁酮。
与瓜实蝇相关的细菌内共生体具有引诱潜力，这有助于制定生态友好型昆虫控制策略。© 2015化学工业协会。
