Zheng Pengfei, Shao Qiang, Diao Xiaoping, Li Zandong, Han Qian
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Agricultural College, Hainan University, Haikou 570228, China.
State Key Laboratory for Agribiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Gene. 2016 Jan 1;575(1):58-65. doi: 10.1016/j.gene.2015.08.034. Epub 2015 Aug 20.
Stem cell pluripotency factors can induce somatic cells to form induced pluripotent stem cells, which are involved in cell reprogramming and dedifferentiation. The tissue regeneration in the earthworm Eisenia foetida may involve cell dedifferentiation. There is limited information about associations between pluripotency factors and the regeneration. In this report, cDNA sequences of pluripotency factors, oct4, nanog, sox2, c-myc and lin28 genes from the earthworm E. foetida were cloned, and quantitative PCR analysis was performed for their mRNA expressions in the head, clitellum and tail. The maximum up-regulation of oct4, nanog, sox2, c-myc and lin28 occurred at 12h, 4 days, 12h, 2 days, and 24h after amputation for 110, 178, 21, 251 and 325-fold, respectively, in comparison with the controls. The results suggest that the tissues are regenerated via cellular dedifferentiation and reprogramming.
干细胞多能性因子可诱导体细胞形成诱导多能干细胞，这一过程涉及细胞重编程和去分化。赤子爱胜蚓的组织再生可能涉及细胞去分化。关于多能性因子与再生之间的关联，目前的信息有限。在本报告中，克隆了赤子爱胜蚓多能性因子oct4、nanog、sox2、c-myc和lin28基因的cDNA序列，并对其在头部、环带和尾部的mRNA表达进行了定量PCR分析。与对照组相比，切断后12小时、4天、12小时、2天和24小时，oct4、nanog、sox2、c-myc和lin28的上调倍数分别为110、178、21、251和325倍。结果表明，组织是通过细胞去分化和重编程实现再生的。
