Sahu Ranjan, Sahu Sanjat Kumar, Nishank Sudhansu Sekhar
Post Graduate Department of Zoology, Utkal University, Vani Vihar, Bhubaneswar, 751004, Odisha, India.
Dept. of Environment Science, Sambalpur University, Jyoti Vihar, Burla, 768019, Odisha, India.
Biochem Biophys Rep. 2021 Aug 9;27:101092. doi: 10.1016/j.bbrep.2021.101092. eCollection 2021 Sep.
Earthworms have remarkable ability to regenerate its tail and head region. However the list of genes expressed in this regeneration process has been less explored baring a few species. The current study involves the transcriptome sequencing of intact tail and regenerating tail (15 day post amputation) of earthworms belonging to two different genera (Kinberg, 1867) and (Gates, 1945). This study contains one and one reference based transcriptome analysis each from one genus of two earthworm genera. From a total of 119.92 million (150 × 2) reads, 112.95 million high quality adapter free reads were utilized in analysis. Assembly of high-quality reads was performed separately for (LM sample) and (DC sample) that resulted in 66368 and 1,61,289 transcripts respectively. About 25.21% of transcripts were functionally annotated for DC sample and 38.27% for LM samples against Annelida sequences. A total of 239 genes were expressed exclusively in regenerated tissue compared to intact sample in DC whereas about 241 genes were exclusively expressed in regenerated tissue of LM compared to its intact sample. Majority of genes in and were dedicated to immune response, maintenance of cytoskeleton, resisting oxidative stress and promoting neuronal regeneration for cell-cell communication during tail regeneration.
蚯蚓具有显著的再生其尾部和头部区域的能力。然而，除了少数物种外，在这个再生过程中表达的基因列表尚未得到充分探索。当前的研究涉及对属于两个不同属（金伯格，1867年）和（盖茨，1945年）的蚯蚓完整尾部和再生尾部（截肢后15天）进行转录组测序。这项研究包含来自两个蚯蚓属中各一个属的基于一个参考的转录组分析。在总共1.1992亿（150×2）条 reads 中，1.1295亿条无接头的高质量 reads 被用于分析。分别对（LM样本）和（DC样本）进行高质量 reads 的组装，结果分别产生了66368条和161289条转录本。针对环节动物序列，DC样本中约25.21%的转录本得到了功能注释，LM样本中为38.27%。与DC中的完整样本相比，共有239个基因仅在再生组织中表达，而与LM的完整样本相比，约241个基因仅在其再生组织中表达。在和中的大多数基因致力于免疫反应、细胞骨架的维持、抵抗氧化应激以及促进尾部再生过程中细胞间通讯的神经元再生。
