Lusseau David, Mitchell Sharon E, Barros Ceres, Derous Davina, Green Cara, Chen Luonan, Han Jing-Dong Jackie, Wang Yingchun, Promislow Daniel E L, Douglas Alex, Speakman John R
Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK Ab24 2TZ.
Key laboratory of Systems Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, 800 Cao Bao road, Shanghai, China.
Sci Rep. 2015 Aug 25;5:13198. doi: 10.1038/srep13198.
Animals have to adjust their activities when faced with caloric restriction (CR) to deal with reduced energy intake. If CR is pronounced, allostasis can push individuals into alternate physiological states which can result in important health benefits across a wide range of taxa. Here we developed a new approach to determine the changes in behavioural phenotype associated with different levels of CR. We exposed C57BL/6 male mice to graded CR (from 0 to 40%) for three months and defined their behavioural phenotype using hidden Markov models of their movement and body temperature. All 40% CR mice exhibited a state-shift in behavioural phenotype and only some exposed to 30% CR did. We show for the first time that mice changed their activity characteristics rather than changed their activities. This new phenotyping approach provides an avenue to determine the mechanisms linking CR to healthspan.
面对热量限制（CR）时，动物必须调整其活动以应对能量摄入的减少。如果热量限制显著，体内平衡可促使个体进入交替的生理状态，这会给广泛的生物分类群带来重要的健康益处。在此，我们开发了一种新方法来确定与不同程度热量限制相关的行为表型变化。我们将C57BL/6雄性小鼠暴露于分级热量限制（从0%至40%）三个月，并使用其运动和体温的隐马尔可夫模型来定义它们的行为表型。所有40%热量限制的小鼠在行为表型上都出现了状态转变，而只有部分暴露于30%热量限制的小鼠出现了这种转变。我们首次表明，小鼠改变的是其活动特征而非活动本身。这种新的表型分析方法为确定将热量限制与健康寿命联系起来的机制提供了一条途径。
