Tanca Alessandro, Palomba Antonio, Pisanu Salvatore, Addis Maria Filippa, Uzzau Sergio
Porto Conte Ricerche, Tramariglio, Alghero, Italy.
Porto Conte Ricerche, Tramariglio, Alghero, Italy ; Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy.
Data Brief. 2015 Jul 26;4:559-62. doi: 10.1016/j.dib.2015.07.014. eCollection 2015 Sep.
We present a human gut metaproteomic dataset deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001573. Ten aliquots of a single stool sample collected from a healthy human volunteer were either pretreated by differential centrifugation (DC; N=5) or not centrifuged (NC; N=5). Protein extracts were then processed by filter-aided sample preparation, single-run liquid chromatography and high-resolution mass spectrometry, and peptide identification was carried out using Sequest-HT as search engine within the Proteome Discoverer informatic platform. The dataset described here is also related to the research article entitled "Enrichment or depletion? The impact of stool pretreatment on metaproteomic characterization of the human gut microbiota" published in Proteomics (Tanca et al., 2015), [1].
我们展示了一个人类肠道宏蛋白质组数据集，该数据集通过PRIDE合作伙伴库存入蛋白质组交换联盟，数据集标识符为PXD001573。从一名健康人类志愿者采集的一份粪便样本的十个等分试样，其中五个经差速离心（DC）预处理，另外五个未进行离心（NC）。然后，蛋白质提取物通过滤膜辅助样品制备、单通道液相色谱和高分辨率质谱进行处理，并在蛋白质组发现者信息平台内使用Sequest-HT作为搜索引擎进行肽段鉴定。此处描述的数据集还与发表在《蛋白质组学》（Tanca等人，2015年）[1]上的题为“富集还是消耗？粪便预处理对人类肠道微生物群宏蛋白质组特征的影响”的研究文章相关。
