Soliman Dominik, Tserevelakis George J, Omar Murad, Ntziachristos Vasilis
Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany; Chair for Biological Imaging, Technische Universität München, Ismaningerstr. 22, 81675 München, Germany.
Sci Rep. 2015 Aug 26;5:12902. doi: 10.1038/srep12902.
Biology requires observations at multiple geometrical scales, a feature that is not typically offered by a single imaging modality. We developed a hybrid optical system that not only provides different contrast modes but also offers imaging at different geometrical scales, achieving uniquely broad resolution and a 1000-fold volume sampling increase compared to volumes scanned by optical microscopy. The system combines optoacoustic mesoscopy, optoacoustic microscopy and two-photon microscopy, the latter integrating second and third harmonic generation modes. Label-free imaging of a mouse ear and zebrafish larva ex-vivo demonstrates the contrast and scale complementarity provided by the hybrid system. We showcase the superior anatomical orientation offered by the label-free capacity and hybrid operation, over fluorescence microscopy, and the dynamic selection between field of view and resolution achieved, leading to new possibilities in biological visualization.
生物学需要在多个几何尺度上进行观察，这一特性通常不是单一成像方式所能提供的。我们开发了一种混合光学系统，它不仅能提供不同的对比度模式，还能在不同的几何尺度上进行成像，与光学显微镜扫描的体积相比，实现了独特的宽分辨率和1000倍的体积采样增加。该系统结合了光声介观显微镜、光声显微镜和双光子显微镜，后者集成了二次和三次谐波产生模式。对小鼠耳朵和斑马鱼幼虫进行的无标记体外成像证明了该混合系统所提供的对比度和尺度互补性。我们展示了无标记能力和混合操作所提供的优于荧光显微镜的优越解剖定位，以及在视野和分辨率之间实现的动态选择，为生物可视化带来了新的可能性。
