Zheng Wei, Wu Yicong, Winter Peter, Fischer Robert, Nogare Damian Dalle, Hong Amy, McCormick Chad, Christensen Ryan, Dempsey William P, Arnold Don B, Zimmerberg Joshua, Chitnis Ajay, Sellers James, Waterman Clare, Shroff Hari
Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA.
Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Nat Methods. 2017 Sep;14(9):869-872. doi: 10.1038/nmeth.4337. Epub 2017 Jun 19.
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ∼250 μm from the coverslip surface.
我们利用非线性导星来确定光学像差，并使用可变形镜来校正像差，从而改进了多光子结构照明显微镜。我们在微珠模型、胶原凝胶中的细胞、线虫幼虫和胚胎、果蝇大脑以及斑马鱼胚胎上展示了我们的方法。在距盖玻片表面约250μm的深度处，峰值强度增加（高达40倍），分辨率得到恢复（横向高达176±10nm，轴向高达729±39nm）。
